Purification and characterization of plantaricin LR14: a novel bacteriocin produced by Lactobacillus plantarum LR/14

被引：0
作者：
Santosh Kumar Tiwari
Sheela Srivastava
机构：
[1] University of Delhi South Campus,Department of Genetics
来源：
Applied Microbiology and Biotechnology | 2008年 / 79卷
关键词：
Bacteriocin; Purification; Cation-exchange chromatography; RP-FPLC; Characterization; Mode of action;
D O I：
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学科分类号：
摘要：
Bacteriocin produced by Lactobacillus plantarum strain LR/14 was purified to homogeneity by a multi-step protocol consisting of ammonium sulfate precipitation, cation-exchange chromatography, gel-filtration, and reverse-phase FPLC. L. plantarum LR/14 secreted a low-molecular-weight bacteriocin consisting of two peptides designated as plantaricin LR14α and -β with molecular mass of 3,012.46 and 5,605.74 Da, respectively. The purified peptides were characterized to be highly thermostable and active in acidic pH range, with a pI of >10.0. Both α and β peptides showed bactericidal mode of action against indicator strain, Micrococcus luteus and together showed a synergistic action. These peptides were differentially sensitive to a range of proteolytic enzymes, indicating differences in their composition. Amino acid sequencing revealed that the N-terminus in both the cases is blocked; thus, only a partial sequence could be obtained after CNBr digestion. These sequences, when compared with those available in the database, showed no homology with known bacteriocins, indicating it to be a novel compound.
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页码：759 / 767
页数：8
相关论文
共 120 条
[11]  
Bauer R(2001) ATCC 9857 from human female genitourinary tract Microbiology 147 643-651
[12]  
Chikindas ML(1995)Biochemical and genetic evidence that Microbiol Rev 59 171-200
[13]  
Dicks LMT(1995) L50 produces enterocins L50A and L50B, the sec-dependent enterocin P, and a novel bacteriocin secreted without an N-terminal extension termed enterocin Q Appl Environ Microbiol 61 4459-4463
[14]  
Blom H(2005)Bacteriocin safe, natural antimicrobials for food preservation J Appl Microbiol 98 881-888
[15]  
Katla T(1993)Two-peptide bacteriocins produced by lactic acid bacteria FEMS Microbiol Rev 12 39-86
[16]  
Holck A(2003)Planaricin W from Appl Environ Microbiol 69 383-389
[17]  
Sletten K(2001) belongs to a new family of two peptide lantibiotics Int J Food Microbiol 64 193-198
[18]  
Axelsson L(2007)Bacteriocins of Gram-positive bacteria J Appl Microbiol 102 274-282
[19]  
Holo H(1998)Purification and partial amino acid sequence of plantaricin S, a bacteriocin produced by J Appl Microbiol 85 603-614
[20]  
Breukik E(2005) LPCO10, the activity of which depends on the complementary action of two peptides Antimicrob Agents Chemother 49 2606-2611
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