Development of two microsatellite multiplex PCR systems for high throughput genotyping in Populus euphratica

被引：9

作者：

Pascal E. ^{[1
]}

Steffen F. ^{[1
]}

Martin S. ^{[1
]}

机构：

[1] Department of Botany and Landscape Ecology, EMAU Greifswald, 17487 Greifswald

来源：

Journal of Forestry Research | 2009年 / 20卷 / 3期

关键词：

Clone identification; Euphrates poplar; Genetic fingerprint; Parentage analysis; Population structure; SSR primers;

D O I：

10.1007/s11676-009-0038-7

中图分类号：

学科分类号：

摘要：

Eighteen microsatellite primer pairs previously developed at Oak Ridge National Laboratory for Populus tremuloides Michx. and Populus trichocarpa Torr. & Gray were screened for amplification in Euphrates poplar, Populus euphratica Oliv. Thirteen loci were found to express polymorphisms ranging from two to 17 alleles. The eight most variable loci were selected to set up and optimize two multiplex polymerase chain reaction (PCR) assays. Three populations containing altogether 436 trees were used to characterize the selected loci and ascertain their applicability for parentage analysis and genotyping studies. Through cross-checking of clonal identity against sex of the genotyped trees we estimated the maximum error rate for merging genotypes to be less than 0.045. © Northeast Forestry University and Springer-Verlag 2009.

引用

页码：195 / 198

页数：3

共 21 条

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