Production of fluorescent and cytotoxic K28 killer toxin variants through high cell density fermentation of recombinant Pichia pastoris

被引:0
|
作者
Esther Giesselmann
Björn Becker
Manfred J. Schmitt
机构
[1] Saarland University,Molecular and Cell Biology, Department of Biosciences and Center of Human and Molecular Biology (ZHMB)
来源
关键词
High cell density fermentation; Fluorescence labelling; Heterologous protein expression; Killer toxin; A/B toxins;
D O I
暂无
中图分类号
学科分类号
摘要
引用
收藏
相关论文
共 50 条
  • [21] Production of enzymatically active recombinant full-length barley high pI α-glucosidase of glycoside family 31 by high cell-density fermentation of Pichia pastoris and affinity purification
    Naested, H
    Kramhoft, B
    Lok, F
    Bojsen, K
    Yu, SK
    Svensson, B
    PROTEIN EXPRESSION AND PURIFICATION, 2006, 46 (01) : 56 - 63
  • [22] A simple fermentation strategy for high-level production of recombinant phytase by Pichia pastoris using glucose as the growth substrate
    Hang, Haifeng
    Ye, Xinhao
    Guo, Meijin
    Chu, Ju
    Zhuang, Yingping
    Zhang, Ming
    Zhang, Siliang
    ENZYME AND MICROBIAL TECHNOLOGY, 2009, 44 (04) : 185 - 188
  • [23] Secretory expression of functional barley limit dextrinase by Pichia pastoris using high cell-density fermentation
    Vester-Christensen, Malene Bech
    Abou Hachem, Maher
    Naested, Henrik
    Svensson, Birte
    PROTEIN EXPRESSION AND PURIFICATION, 2010, 69 (01) : 112 - 119
  • [24] High efficient production of recombinant human consensus interferon mutant in high cell density culture of Pichia pastoris using two phases methanol control
    Wu, Dan
    Chu, Ju
    Hao, Yu-You
    Wang, Yong-Hong
    Zhuang, Ying-Ping
    Zhang, Si-Liang
    PROCESS BIOCHEMISTRY, 2011, 46 (08) : 1663 - 1669
  • [25] Expression of a novel regenerating gene product, Reg IV, by high density fermentation in Pichia pastoris:: production, purification, and characterization
    Li, A
    Crimmins, DL
    Luo, Q
    Hartupee, J
    Landt, Y
    Ladenson, JH
    Wilson, D
    Anant, S
    Dieckgraefe, BK
    PROTEIN EXPRESSION AND PURIFICATION, 2003, 31 (02) : 197 - 206
  • [26] High-yield production of the human lysozyme by Pichia pastoris SMD1168 using response surface methodology and high-cell-density fermentation
    Yu, Ying
    Zhou, Xiaoyu
    Wu, Sheng
    Wei, Tiantian
    Yu, Long
    ELECTRONIC JOURNAL OF BIOTECHNOLOGY, 2014, 17 (06): : 311 - 316
  • [27] Enhanced recombinant human erythropoietin production by Pichia pastoris in methanol fed-batch/sorbitol batch fermentation through pH optimization
    Soyaslan, Elif Sukran
    Calik, Pinar
    BIOCHEMICAL ENGINEERING JOURNAL, 2011, 55 (01) : 59 - 65
  • [28] Efficient secretory expression of functional barley limit dextrinase inhibitor by high cell-density fermentation of Pichia pastoris
    Jensen, Johanne Morch
    Vester-Christensen, Malene Bech
    Moller, Marie Sofie
    Bonsager, Birgit C.
    Christensen, Hans Erik Molager
    Abou Hachem, Maher
    Svensson, Birte
    PROTEIN EXPRESSION AND PURIFICATION, 2011, 79 (02) : 217 - 222
  • [29] Recovery of recombinant β-glucosidase by expanded bed adsorption from Pichia pastoris high-cell-density culture broth
    Charoenrat, T
    Ketudat-Cairns, M
    Jahic, M
    Enfors, SO
    Veide, A
    JOURNAL OF BIOTECHNOLOGY, 2006, 122 (01) : 86 - 98
  • [30] High level production and purification of human interferon α-2b in high cell density culture of Pichia pastoris
    Ayed, Atef
    Rabhi, Imen
    Dellagi, Koussay
    Kallel, Hela
    ENZYME AND MICROBIAL TECHNOLOGY, 2008, 42 (02) : 173 - 180