Physical, chemical, and regulatory properties of glycolate oxidase in C3 and C4 plants

被引:0
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作者
A. T. Eprintsev
A. E. Semenov
M. Navid
V. N. Popov
机构
[1] Voronezh State University,Department of Plant Physiology and Biochemistry, Faculty of Biology and Soil Science
来源
Russian Journal of Plant Physiology | 2009年 / 56卷
关键词
Zea mays; Glycine max; glycolate oxidase; mesophyll; cortex; electrophoresis; gel chromatography;
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学科分类号
摘要
Physical, chemical, and regulatory properties of glycolate oxidase (GO) isolated from the leaves of C4 and C3 plants (Zea mays L., cv. Voronezhskaya 76 and Glycine max (L.) Merr., cv. Pripyat’, respectively) were studied. The homogenous preparations were obtained by multistage enzyme purification from soybean leaves and maize mesophyll and bundle sheath. The glycolate oxidase (GO) preparations obtained consisted of two types of subunits, 37 and 44 kD. The GO isolated from C3 plant leaves had many in common with that extracted from C4 plant bundle sheath as regards physical, chemical, and catalytic properties. The primary function of GO in both plant types is metabolism of glycolate, which is a product of ribulosebisphosphate oxalacetic acid oxidation and is used by plants for biosynthesis of hydrocarbons and amino acids.
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页码:164 / 167
页数:3
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