Antioxidant and anti-inflammatory properties of C-phycocyanin from blue-green algae

被引：0

作者：

C. Romay

J. Armesto

D. Remirez

R. González

N. Ledon

I. García

机构：

[1] Pharmacology Department,

[2] National Center for Scientific Research,undefined

[3] CNIC,undefined

[4] P.O. Box 6990,undefined

[5] Havana,undefined

[6] Cuba,undefined

[7] Fax +53 7 330497,undefined

[8] e-mail: ricardo@quimica.cneuro.cu,undefined

[9] Pharmacy and Foods Faculty,undefined

[10] University of Havana,undefined

[11] Ave. 23,undefined

[12] No. 21425,undefined

[13] La Coronela,undefined

[14] Havana,undefined

[15] Cuba,undefined

来源：

Inflammation Research | 1998年 / 47卷

关键词：

Key words: Antioxidant — Chemiluminescence — C-Phycocyanin — Blue-green algae — Free radical scavenger;

D O I：

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学科分类号：

摘要：

Objective: Phycocyanin is a pigment found in blue-green algae which contains open chain tetrapyrroles with possible scavenging properties. We have studied its antioxidant properties.¶Materials and methods: Phycocyanin was evaluated as a putative antioxidant in vitro by using: a) luminol-enhanced chemiluminescence (LCL) generated by three different radical species (O−2, OH•, RO•) and by zymosan activated human polymorphonuclear leukocytes (PMNLs), b) deoxyribose assay and c) inhibition of liver microsomal lipid peroxidation induced by Fe+2-ascorbic acid. The antioxidant activity was also assayed in vivo in glucose oxidase (GO)-induced inflammation in mouse paw.¶Results: The results indicated that phycocyanin is able to scavenge OH• (IC50 = 0.91 mg/mL) and RO• (IC50 = 76 μg/mL) radicals, with activity equivalent to 0.125 mg/mL of dimethyl sulphoxide (DMSO) and 0.038 μg/mL of trolox, specific scavengers of those radicals respectively. In the deoxyribose assay the second-order rate constant was 3.56 × 1011 M−1 S−1, similar to that obtained for some non-steroidal anti-inflammatory drugs. Phycocyanin also inhibits liver microsomal lipid peroxidation (IC50 = 12 mg/mL), the CL response of PMNLs (p < 0.05) as well as the edema index in GO-induced inflammation in mouse paw (p < 0.05).¶Conclusions: To our knowledge this is the first report of the antioxidant and anti-inflammatory properties of c-phycocyanin.

引用

页码：36 / 41

页数：5