Biocompatibility of vertically aligned multi-walled carbon nanotube scaffolds for human breast cancer cell line MDA-MB-231

被引：13

作者：

Akinoglu E.M. ^{[1
,2
]}

Ozbilgin K. ^{[3
,4
]}

Sonmez P.K. ^{[3
]}

Ozkut M.M. ^{[3
]}

Giersig M. ^{[1
,2
]}

Inan S. ^{[5
]}

Gumustepe E. ^{[6
]}

Kurtman C. ^{[6
]}

机构：

[1] International Academy of Optoelectronics at Zhaoqing, South China Normal University, Zhaoqing, Guangdong

[2] Department of Physics, Freie Universität Berlin, Berlin

[3] Department of Histology and Embryology, Faculty of Medicine, Celal Bayar University, Uncubozköy, Manisa

[4] Department of Histology and Embryology, Faculty of Medicine, Near East University, Manisa

[5] Department of Histology and Embryology, Faculty of Medicine, Izmir University of Economy, Izmir

[6] Department of Radiation Oncology, Faculty of Medicine, Ankara University, Ankara

来源：

Progress in Biomaterials | 2017年 / 6卷 / 4期

关键词：

Biocompatibility; Breast cancer; Immunohistochemistry; MDA-MB-231; MWCNTs;

D O I：

10.1007/s40204-017-0078-6

中图分类号：

学科分类号：

摘要：

The aim of the current study was to determine whether the MWCNT-based scaffold has a suitable structure for cell growth and provides a biocompatible environment for human MDA-MB-231 cell lines. MWCNT-based nanostructured scaffolds were produced by plasma-enhanced chemical vapor deposition (PECVD) technique. MDA-MB-231 cells were seeded on MWCNTs-textured silicon scaffolds and on pristine silicon surfaces. After 1 week of culturing, the scaffolds were prepared for SEM analysis and immunocytochemical staining was performed for the two groups (MWCNT scaffold and pristine silicon surface), using MMP-2, MMP-9, PI3K, AKT and NF-κB primary antibodies. SEM analyses showed that the MDA-MB-231 cells better adhered to the MWCNT-based nanostructured scaffold than the pristine silicon surface. Immunohistochemical activity of the MDA-MB-231 cells on both materials has similar staining with anti-AKT MMP-2, MMP-9 and NF-κB primary antibodies. Therefore, the results of the present study suggest that the MWCNT-based scaffolds enhanced cell adhesion to the scaffold and exhibited more biomimetic properties and physiological adaptation with the potential to be used for in vitro metastasis studies of BrCa cell lines. © 2017, The Author(s).

引用

页码：189 / 196

页数：7

共 30 条

[1]

Adeyinka A., Nui Y., Cherlet T., Snell L., Watson P.H., Murphy L.C., Activated mitogen-activated protein kinase expression during human breast tumorigenesis and breast cancer progression, Clin Cancer Res, 8, pp. 1747-1753, (2002)

[2]

Baselga J., Targeting the phosphoinositide-3 (PI3) kinase pathway in breast cancer, Oncologist, 16, pp. 12-19, (2011)

[3]

Britten C.D., PI3K, MEK inhibitor combinations: examining the evidence in selected tumor types, Cancer Chemother Pharmacol, 71, pp. 1395-1409, (2013)

[4]

Cizkova M., Cizeron-Clairac G., Vacher S., Susini A., Andrieu C., Lidereau R., Et al., Gene expression profiling reveals new aspects of PIK3CA mutation in ERalpha-positive breast cancer: major implication of the Wnt signaling pathway, PLoS One, 5, (2010)

[5]

Correa-Duarte M.A., Wagner N., Rojas-Chapana J., Morsczeck C., Thie M., Giersig M., Fabrication and biocompatibility of carbon nanotube-based 3D networks as scaffolds for cell seeding and growth, Nano Lett, 4, pp. 2233-2236, (2004)

[6]

deGraffenried L.A., Friedrichs W.E., Russell D.H., Donzis E.J., Middleton A.K., Silva J.M., Et al., Inhibition of mTOR activity restores tamoxifen response in breast cancer cells with aberrant Akt Activity, Clin Cancer Res, 10, pp. 8059-8067, (2004)

[7]

Deryugina E.I., Quigley J.P., Matrix metalloproteinases and tumor metastasis, Cancer Metastasis Rev, 25, pp. 9-34, (2006)

[8]

Firkowska I., Olek M., Pazos-Perez N., Rojas-Chapana J., Giersig M., Highly ordered MWNT-based matrixes: topography at the nanoscale conceived for tissue engineering, Langmuir, 22, pp. 5427-5434, (2006)

[9]

Firkowska I., Giannona S., Rojas-Chapana J., Luecke K., Brustle O., Giersig M., Biocompatible nanomaterials and nanodevices promising for biomedical applications, Nanomaterials for application in medicine and biology, pp. 1-15, (2008)

[10]

Hoeflich K.P., O'Brien C., Boyd Z., Cavet G., Guerrero S., Jung K., Et al., In vivo antitumor activity of MEK and phosphatidylinositol 3-kinase inhibitors in basal-like breast cancer models, Clin Cancer Res, 15, pp. 4649-4664, (2009)

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