Use of loop-mediated isothermal amplification of DNA for the rapid detection of Mycobacterium tuberculosis in clinical specimens

被引:0
作者
I. K. Neonakis
D. A. Spandidos
E. Petinaki
机构
[1] University of Thessaly,Department of Microbiology, Medical School
[2] University Hospital of Heraklion,Department of Microbiology
[3] University of Crete,Department of Laboratory Medicine, Medical School
来源
European Journal of Clinical Microbiology & Infectious Diseases | 2011年 / 30卷
关键词
Tuberculosis; Calcein; Sputum Sample; Lamp Assay; Lamp Reaction;
D O I
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中图分类号
学科分类号
摘要
Loop-mediated isothermal amplification (LAMP) is a recently developed molecular method that has been successfully implemented in the detection of Mycobacterium tuberculosis in clinical specimens. LAMP has several advantages, such as rapidity, high sensitivity, ease of application and cost-effectiveness. As a result, it is anticipated that its use for the detection of M. tuberculosis is likely to become widespread, especially in low-resource countries. The present review aimed to present this method and all of the available information on its implementation in the detection of M. tuberculosis in clinical specimens.
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页码:937 / 942
页数:5
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