Epigenetic mechanisms of peptidergic regulation of gene expression during aging of human cells

被引：0
作者：
V. V. Ashapkin
N. S. Linkova
V. Kh. Khavinson
B. F. Vanyushin
机构：
[1] Lomonosov Moscow State University,Belozersky Institute of Physico
[2] St. Petersburg Institute of Bioregulation and Gerontology,Chemical Biology
[3] St. Petersburg State Polytechnical University,undefined
[4] Mechnikov North-West State Medical University,undefined
来源：
Biochemistry (Moscow) | 2015年 / 80卷
关键词：
short peptides; DNA methylation; transcription; cell differentiation; pancreatic cell culture; bronchial cell culture; differentiation factors;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Expression levels of genes encoding specific transcription factors and other functionally important proteins vary upon aging of pancreatic and bronchial epithelium cell cultures. The peptides KEDW and AEDL tissue-specifically affect gene expression in pancreatic and bronchial cell cultures, respectively. It is established in this work that the DNA methylation patterns of the PDX1, PAX6, NGN3, NKX2-1, and SCGB1A1 gene promoter regions change upon aging in pancreatic and bronchial cell cultures in correlation with variations in their expression levels. Thus, stable changes in gene expression upon aging of cell cultures could be caused by changes in their promoter methylation patterns. The methylation patterns of the PAX4 gene in pancreatic cells as well as those of the FOXA1, SCGB3A2, and SFTPA1 genes in bronchial cells do not change upon aging and are unaffected by peptides, whereas their expression levels change in both cases. The promoter region of the FOXA2 gene in pancreatic cells contains a small number of methylated CpG sites, their methylation levels being affected by cell culture aging and KEDW, though without any correlation with gene expression levels. The promoter region of the FOXA2 gene is completely unmethylated in bronchial cells irrespective of cell culture age and AEDL action. Changes in promoter methylation might be the cause of age- and peptide-induced variations in expression levels of the PDX1, PAX6, and NGN3 genes in pancreatic cells and NKX2-1 and SCGB1A1 genes in bronchial cells. Expression levels of the PAX4 and FOXA2 genes in pancreatic cells and FOXA1, FOXA2, SCGB3A2, and SFTPA1 genes in bronchial cells seem to be controlled by some other mechanisms.
引用
收藏
页码：310 / 322
页数：12
相关论文
共 159 条
  • [11] Vasilyev A V(2005)Cross-sectional and longitudinal changes in DNA methylation with age: an epigenome-wide analysis revealing over 60 novel age-associated CpG sites Nucleic Acids Res. 33 e9-13
  • [12] Ryzhak G A(2006)BiSearch: primer-design and search tool for PCR on bisulfite treated genomes BMC Bioinformatics 7 431-414
  • [13] Korkushko O V(2000)The BiSearch web server Nucleic Acids Res. 28 1053-244
  • [14] Khavinson V Kh(2004)MethTools — a toolbox to visualize and analyze DNA methylation data Bioinformatics 20 3005-1720
  • [15] Shatilo V B(2004)Quantitative DNA methylation analysis based on four-dye trace data from direct sequencing of PCR amplificates PLoS Biol. 2 e405-1022
  • [16] Antonyk-Sheglova I A(2013)DNA methylation profiling of the human major histocompatibility complex: a pilot study for the human epigenome project Devel. Cell 25 5-492
  • [17] Korkushko O V(2014)Gene regulatory networks governing pancreas development Trends Endocrinol. Metab. 25 407-220
  • [18] Khavinson V Kh(2007)Revealing transcription factors during human pancreatic β cell development Physiol. Rev. 87 219-738
  • [19] Shatilo V B(2009)Transcriptional control of lung morphogenesis FEBS Lett. 583 1713-4310
  • [20] Antonyk-Sheglova I A(2011)CpG islands — “a rough guide” Genes Devel. 25 1010-3072
12345678910