Microsatellite markers of Gonystylus bancanus (Thymelaeaceae) for population genetic studies and DNA fingerprinting

被引：0
|
作者：
Kevin Kit Siong Ng
Soon Leong Lee
Chin Hong Ng
Lee Hong Tnah
Chai Ting Lee
Naoki Tani
机构：
[1] Forest Research Institute Malaysia,Genetic Laboratory
[2] Japan International Research Center for Agricultural Sciences,Forestry Division
来源：
Conservation Genetics Resources | 2009年 / 1卷
关键词：
DNA fingerprinting; Genomic DNA library; Microsatellite; Ramin; Peat swamp forest;
D O I：
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学科分类号：
摘要：
This paper reports 10 polymorphic microsatellite markers for a valuable peat swamp timber species, Gonystylus bancanus (Thymelaeaceae). The primers were designed from a genomic library enriched with dinucleotide (CT) repeats and screened on 24 samples from a natural population. The number of alleles detected per locus ranged from two to nine while the observed and expected heterozygosities ranged from 0.455 to 0.864 and from 0.476 to 0.863, respectively. No significant deviation from Hardy–Weinberg equilibrium (P < 0.05) was detected in all the 10 loci. These markers are currently being applied on population genetic studies and DNA fingerprinting databases establishment for individual identification of G. bancanus in Malaysia.
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