Gene-trap mutagenesis: past, present and beyond

被引：0
作者：
William L. Stanford
Jason B. Cohn
Sabine P. Cordes
机构：
[1] Programme in Development and Fetal Health,Department of Molecular and Medical Genetics
[2] Samuel Lunenfeld Research Institute,undefined
[3] Mount Sinai Hospital,undefined
[4] University of Toronto,undefined
来源：
Nature Reviews Genetics | 2001年 / 2卷
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摘要：
Although at least 35,000 human genes have been sequenced and mapped, adequate expression or functional information is available for only ∼15% of them. Gene-trap mutagenesis is a technique that randomly generates loss-of-function mutations and reports the expression of many mouse genes. At present, several large-scale, gene-trap screens are being carried out with various new vectors, which aim to generate a public resource of mutagenized embryonic stem (ES) cells. This resource now includes more than 8,000 mutagenized ES-cell lines, which are freely available, making it an appropriate time to evaluate the recent advances in this area of genomic technology and the technical hurdles it has yet to overcome.
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页码：756 / 768
页数：12
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