Separation of long-range human TERT gene haplotypes by transformation-associated recombination cloning in yeast

被引：0
|
作者：
Jung-Hyun Kim
Sun-Hee Leem
Yangil Sunwoo
Natalay Kouprina
机构：
[1] Laboratory of Biosystems and Cancer,Department of Biology
[2] National Cancer Institute (NIH),undefined
[3] Dong-A University,undefined
来源：
Oncogene | 2003年 / 22卷
关键词：
hTERT; VNTR; polymorphism; haplotypes; TAR cloning;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The hTERT gene encoding a catalytic subunit of human telomerase contains four blocks of variable number of tandem repeats (VNTRs) – two in intron 2 and two in intron 6. The segregation of hTERT VNTRs was analysed in families, revealing that all of them were transmitted through meiosis following a Mendelian inheritance. The work reports a further characterization of the minisatellites in hTERT. We employed transformation-associated recombination (TAR) cloning to isolate parental hTERT alleles and determined the specific combination of minisatellites at each of the polymorphic sites. A long-range haplotyping of hTERT determined by TAR cloning was verified by classical Mendelian analysis. Since such a strategy can be applied for any chromosomal locus, we conclude that recombinational gene capture could greatly facilitate haplotypes analysis.
引用
收藏
页码：2452 / 2456
页数：4
相关论文
共 50 条
  • [1] Separation of long-range human TERT gene haplotypes by transformation-associated recombination cloning in yeast
    Kim, JH
    Leem, SH
    Sunwoo, Y
    Kouprina, N
    ONCOGENE, 2003, 22 (16) : 2452 - 2456
  • [2] Specific cloning of human DNA as yeast artificial chromosomes by transformation-associated recombination
    Larionov, V
    Kouprina, N
    Graves, J
    Chen, XN
    Korenberg, JR
    Resnick, MA
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (01) : 491 - 496
  • [3] Cloning of human centromeres by transformation-associated recombination in yeast and generation of functional human artificial chromosomes
    Kouprina, N
    Ebersole, T
    Koriabine, M
    Pak, E
    Rogozin, IB
    Katoh, M
    Oshimura, M
    Ogi, K
    Peredelchuk, M
    Solomon, G
    Brown, W
    Barrett, JC
    Larionov, V
    NUCLEIC ACIDS RESEARCH, 2003, 31 (03) : 922 - 934
  • [4] Direct isolation of human BRCA2 gene by transformation-associated recombination in yeast
    Larionov, V
    Kouprina, N
    Solomon, G
    Barrett, JC
    Resnick, MA
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (14) : 7384 - 7387
  • [5] Isolation of a functional copy of the human BRCA1 gene by transformation-associated recombination in yeast
    Annab, LA
    Kouprina, N
    Solomon, G
    Cable, PL
    Hill, DE
    Barrett, JC
    Larionov, V
    Afshari, CA
    GENE, 2000, 250 (1-2) : 201 - 208
  • [6] Rapid cloning of mouse DNA as yeast artificial chromosomes by transformation-associated recombination (TAR)
    Cancilla, MR
    Graves, J
    Matesic, LE
    Reeves, RH
    Tainton, KM
    Choo, KHA
    Resnick, MA
    Larionov, VL
    Kouprina, NY
    MAMMALIAN GENOME, 1998, 9 (02) : 157 - 159
  • [7] Rapid cloning of mouse DNA as yeast artificial chromosomes by transformation-associated recombination (TAR)
    Cancilla M.R.
    Graves J.
    Matesic L.E.
    Reeves R.H.
    Tainton K.M.
    Choo K.H.A.
    Resnick M.A.
    Larionov V.L.
    Kouprina N.Y.
    Mammalian Genome, 1998, 9 (2) : 157 - 159
  • [8] Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination
    Vashee, Sanjay
    Stockwell, Timothy B.
    Alperovich, Nina
    Denisova, Evgeniya A.
    Gibson, Daniel G.
    Cady, Kyle C.
    Miller, Kristofer
    Kannan, Krishna
    Malouli, Daniel
    Crawford, Lindsey B.
    Voorhies, Alexander A.
    Bruening, Eric
    Caposio, Patrizia
    Fruh, Klaus
    MSPHERE, 2017, 2 (05):
  • [9] Segments missing from the draft human genome sequence can be isolated by transformation-associated recombination cloning in yeast
    Kouprina, N
    Leem, SH
    Solomon, G
    Ly, A
    Koriabine, M
    Otstot, J
    Pak, E
    Dutra, A
    Zhao, SY
    Barrett, JC
    Larionov, V
    EMBO REPORTS, 2003, 4 (03) : 257 - 262
  • [10] Direct cloning of human 10q25 neocentromere DNA using transformation-associated recombination (TAR) in yeast
    Cancilla, MR
    Tainton, KM
    Barry, AE
    Larionov, V
    Kouprina, N
    Resnick, MA
    Du Sart, D
    Choo, KHA
    GENOMICS, 1998, 47 (03) : 399 - 404
12345