scifi-ATAC-seq: massive-scale single-cell chromatin accessibility sequencing using combinatorial fluidic indexing

被引：2

作者：

Zhang, Xuan ^{[1
]}

Marand, Alexandre P. ^{[1
,2
]}

Yan, Haidong ^{[1
]}

Schmitz, Robert J. ^{[1
]}

机构：

[1] Univ Georgia, Dept Genet, Athens, GA 30602 USA

[2] Univ Michigan, Dept Mol Cellular & Dev Biol, Ann Arbor, MI USA

来源：

GENOME BIOLOGY | 2024年 / 25卷 / 01期

关键词：

Single-cell; ATAC-seq; Chromatin accessibility; Combinatorial fluidic indexing; Massive-scale; MAIZE; ATLAS;

D O I：

10.1186/s13059-024-03235-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Single-cell ATAC-seq has emerged as a powerful approach for revealing candidate cis-regulatory elements genome-wide at cell-type resolution. However, current single-cell methods suffer from limited throughput and high costs. Here, we present a novel technique called scifi-ATAC-seq, single-cell combinatorial fluidic indexing ATAC-sequencing, which combines a barcoded Tn5 pre-indexing step with droplet-based single-cell ATAC-seq using the 10X Genomics platform. With scifi-ATAC-seq, up to 200,000 nuclei across multiple samples can be indexed in a single emulsion reaction, representing an approximately 20-fold increase in throughput compared to the standard 10X Genomics workflow.

引用

页数：13

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