Study of the influence of chemical modification of the tryptophan residues in the molecules of Datura innoxia lectins on their hemagglutinating activity

被引：0

作者：

Levitskaya S.V. ^{[1
]}

Yunusov T.S. ^{[1
]}

机构：

[1] Inst. Chem. of Plant Substances, Acad. Sci. of the Rep. of Uzbekistan, Tashkent

来源：

Chemistry of Natural Compounds | 1997年 / 33卷 / 5期

关键词：

Urea; Organic Chemistry; Tryptophan; Chemical Modification; Tryptophan Residue;

D O I：

10.1007/BF02254808

中图分类号：

学科分类号：

摘要：

It has been found that in Datura innoxia lectins 21% of the tryptophan residues are selectively oxidized by N-bromosuccinimide (NBS) under nondenaturing conditions, which shows that one or two residues are exposed on the surface of the molecule. At the same time the hemagglutinating activity (HA) decreases by 50%. When half of all the Trp is oxidized only 30% of the HA is retained. A sharp fall in the HA on the oxidation of ∼1/7 of all the Trp indicates the great importance of at least one residue for the interaction of the lectin with erythrocytes. In the hydrophobic region of the molecule 79% of the Trp is screened, and this is exposed in 8 M urea.

引用

页码：574 / 577

页数：3

共 14 条

[1]

Ashford D., Menon R., Allen A.K., Neuberger A., Biochem. J., 199, (1981)

[2]

Privat J.P., Lotan R., Bouchard P., Sharon N., Monsigny M., Eur. J. Biochem., 68, (1976)

[3]

Datta P.K., Figuerosa M.O.R., Lajolo F.M., J. Agric. Food Chem., 41, (1993)

[4]

Levitskaya S.V., Yunusov T.S., Khim. Prir. Soedin., (1985)

[5]

Spande T.F., Witkop B., Methods Enzymol., 11, (1967)

[6]

Spande T.F., Witkop B., Methods Enzymol., 11, (1967)

[7]

Ramachandran L.K., Witkop B., J. Am. Chem. Soc., 81, (1959)

[8]

Quiocho F.A., O'Dell M., Friedberg F., Experientia, 17, (1961)

[9]

Simpson R.J., Neuberger M.R., Liu T.-Y., J. Biol. Chem., 251, (1976)

[10]

Liu T.-Y., Methods Enzymol., 25, (1972)

← 1 2 →