Partial purification of α-amylase from culture supernatant of Bacillus subtilis in aqueous two-phase systems

被引：0
作者：
Wenbo Zhi
Jiangnan Song
Jingxiu Bi
Fan Ouyang
机构：
[1] Chinese Academy of Sciences,National Key Laboratory of Biochemical Engineering, Institute of Process Engineering
来源：
Bioprocess and Biosystems Engineering | 2004年 / 27卷
关键词：
α-Amylase; Aqueous two-phase systems; Purification;
D O I：
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学科分类号：
摘要：
A study was made of the partition and purification of α-amylase from a culture supernatant of Bacillus subtilis in the polyethylene glycol (PEG)—citrate aqueous two-phase system (ATPS). Factors that influenced the partition of the protein in this system, including the molecular weight of the PEG, the tie line length of ATPS, the pH value and the sodium chloride concentration, were investigated. Purification of α-amylase was attained with a purification factor (PF) of 1.8 and 90% yield at pH 6.0 in a PEG1000-citrate ATPS with short tie line length. By utilizing the salt-out effect of neutral salt, the purification of α-amylase was further improved to 2.0 of PF and 80% yield in a PEG3350-citrate ATPS with 4% sodium chloride.
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页码：3 / 7
页数：4
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