Molecular characterization of Acidithiobacillus ferrooxidans and A. thiooxidans strains isolated from mine wastes in Brazil

被引：1

作者：

Paulino L.C. ^{[1
]}

Bergamo R.F. ^{[1
]}

De Mello M.P. ^{[1
]}

Garcia O. ^{[1
]}

Jr. ^{[1
]}

Manfio G.P. ^{[1
]}

Ottoboni L.M.M. ^{[1
]}

机构：

[1] Ctr. Biol. Mole. Engenharia Genet., Universidade Estadual de Campinas, Campinas SP

来源：

Antonie van Leeuwenhoek | 2001年 / 80卷 / 1期

关键词：

16S rDNA sequence analysis; Acidithiobacillus ferrooxidans; Acidithiobacillus thiooxidans; DNA-DNA homology; Genetic diversity; Rep-PCR; Ribotyping;

D O I：

10.1023/A:1012247325537

中图分类号：

学科分类号：

摘要：

Nineteen strains of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans, including 12 strains isolated from coal, copper, gold and uranium mines in Brazil, strains isolated from similar sources in other countries and the type strains of the two species were characterized together with the type strain of A. caldus by using a combination of molecular systematic methods, namely ribotyping, BOX- and ERIC-PCR and DNA-DNA hybridization assays. Data derived from the molecular fingerprinting analyses showed that the tested strains encompassed a high degree of genetic variability. Two of the Brazilian A. ferrooxidans organisms (strains SSP and PCE) isolated from acid coal mine waste and uranium mine effluent, respectively, and A. thiooxidans strain DAMS, isolated from uranium mine effluent, were the most genetically divergent organisms. The DNA-DNA hybridization data did not support the allocation of Acidithiobacillus strain SSP to the A. ferrooxidans genomic species, as it shared only just over 40% DNA relatedness with the type strain of the species. Acidithiobacillus strain SSP was not clearly related to A. ferrooxidans in the 16S rDNA tree.

引用

页码：65 / 75

页数：10

共 39 条

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