Sandwich immunoassay for the prostate specific antigen using a micro-fluxgate and magnetic bead labels

被引：0
作者：
Xue-cheng Sun
Chong Lei
Lei Guo
Yong Zhou
机构：
[1] Key Laboratory for Thin Film and Microfabrication of the Ministry of Education,
[2] Department of Micro/Nano Electronics,undefined
[3] School of electronic information and electrical engineering,undefined
[4] Shanghai Jiao Tong University,undefined
来源：
Microchimica Acta | 2016年 / 183卷
关键词：
PSA; Dynabeads; Magnetic field sensor; DC magnetic field; Inductive fringing; FeNi permalloy; Self-assembled monolayer;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We describe a micro fluxgate based device with rectangular magnetic core for the determination of prostate specific antigen (PSA) labeled with Dynabeads. A sandwich immunoassay was employed where PSA is captured on a gold film modified with a self-assembled monolayer of antibody. The secondary antibody is labeled with Dynabeads. By applying a DC magnetic fields in the range of 460 to 700 μT, PSA can be detected with detection limit as low as 0.1 ng mL−1. This micro fluxgate-based assay offers the advantages of miniaturization, simple and conveniently manipulation, re-usability and stability. In our perception, it offers a viable approach towards clinical determination of PSA or other biomarkers.
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页码：2385 / 2393
页数：8
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