Adult rat spinal cord culture on an organosilane surface in a novel serum-free medium

被引：0
作者：
Mainak Das
Neelima Bhargava
Cassie Gregory
Lisa Riedel
Peter Molnar
James J. Hickman
机构：
[1] University of Central Florida,Nanoscience Technology Center
[2] Clemson University,Department of Bioengineering
来源：
In Vitro Cellular & Developmental Biology - Animal | 2005年 / 41卷
关键词：
adult rat spinal cord; cell culture; motoneuron; silane surface; serum-free medium; electrophysiology;
D O I：
暂无
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学科分类号：
摘要：
In this study, we have documented by morphological analysis, immunocytochemistry, and electrophysiology, the development of a culture system that promotes the growth and long-term survival of dissociated adult rat spinal cord neurons. This system comprises a patternable, nonbiological, cell growth-promoting organosilane substrate coated on a glass surface and an empirically derived novel serum-free medium, supplemented with specific growth factors (acidic fibroblast growth factor, heparin sulfate, neurotrophin-3, brain-derived neurotrophic factor, glial-derived neurotrophic factor, cardiotrophin-1, and vitronectin). Neurons were characterized by immunoreactivity for neurofilament 150, neuron-specific enolase, Islet-1 antibodies, electrophysiology, and the cultures were maintained for 4–6 wk. This culture system could be a useful tool for the study of adult mammalian spinal neurons in a functional in vitro system.
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页码：343 / 348
页数：5
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