Detection and Characteristics of Rifampicin-Resistant Isolates of Mycobacterium tuberculosis

被引：0
作者：
A. G. Cherednichenko
M. A. Dymova
O. A. Solodilova
T. I. Petrenko
A. I. Prozorov
M. L. Filipenko
机构：
[1] Ministry of Health of the Russian Federation,Research Institute of Tuberculosis
[2] Siberian Division of the Russian Academy of Sciences,Institute of Chemical Biology and Fundamental Medicine
[3] Novosibirsk State University,undefined
[4] Altai Regional Antituberculous Dispensary,undefined
来源：
Bulletin of Experimental Biology and Medicine | 2016年 / 160卷
关键词：
drug resistance; rifampicin; rpoB gene;
D O I：
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摘要：
Genotyping and analysis the drug resistance of 59 isolates of M. tuberculosis obtained from patients living in Altai Territory were performed using a BACTEC MGIT 960 fluorometric system by means of VNTR typing (variable number tandem repeat), PCR-RFLP analysis, and sequence analysis. The occurrence frequency was highest for isolates of the Beijing family (n=30, 50.8%). Analysis of mutation spectrum in the rpoB gene associated with rifampicin resistance revealed the major mutation (codon 531 of the rpoB gene) in 93% samples, which allows us to use rapid test systems.
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页码：659 / 663
页数：4
相关论文
共 18 条
  • [1] Kocagöz T(1993)undefined J. Clin. Microbiol. 31 1435-1438
  • [2] Yilmaz E(2008)undefined Infect. Genet. Evol. 8 777-785
  • [3] Ozkara S(1998)undefined Tuber. Lung Dis. 79 3-29
  • [4] Mokrousov I(1998)undefined Emerg. Infect. Dis. 4 195-209
  • [5] Ramaswamy S(2006)undefined J. Clin. Microbiol. 44 4498-4510
  • [6] Musser JM(1993)undefined J. Clin. Microbiol. 31 406-409
  • [7] Rattan A(2010)undefined Nucleic Acids Res. 38 W326-331
  • [8] Kalia A(undefined)undefined undefined undefined undefined-undefined
  • [9] Ahmad N(undefined)undefined undefined undefined undefined-undefined
  • [10] Supply P(undefined)undefined undefined undefined undefined-undefined
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