Slow nucleic acid unzipping kinetics from sequence-defined barriers

被引：0

作者：

S. Cocco

J.F. Marko

R. Monasson

机构：

[1] CNRS-Laboratoire de Dynamique des Fluides Complexes,

[2] 3 rue de l'Université,undefined

[3] 67000 Strasbourg,undefined

[4] France,undefined

[5] Department of Physics,undefined

[6] The University of Illinois at Chicago,undefined

[7] 845 West Taylor Street,undefined

[8] Chicago,undefined

[9] IL 60607-7059,undefined

[10] USA,undefined

[11] CNRS-Laboratoire de Physique Théorique de l'ENS,undefined

[12] 24 rue Lhomond,undefined

[13] 75005 Paris,undefined

[14] France,undefined

[15] CNRS-Laboratoire de Physique Théorique,undefined

[16] 3 rue de l'Université,undefined

[17] 67000 Strasbourg,undefined

[18] France,undefined

来源：

The European Physical Journal E | 2003年 / 10卷

关键词：

PACS. 87.15.-v Biomolecules: structure and physical properties;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Recent experiments on unzipping of RNA helix-loop structures by force have shown that ≈40-base molecules can undergo kinetic transitions between two well-defined “open” and “closed” states, on a timescale ≈1 sec [Liphardt et al., Science 297, 733-737 (2001)]. Using a simple dynamical model, we show that these phenomena result from the slow kinetics of crossing large free energy barriers which separate the open and closed conformations. The dependence of barriers on sequence along the helix, and on the size of the loop(s) is analyzed. Some DNA and RNA sequences that could show dynamics on different time scales, or three(or more)-state unzipping, are proposed. Our dynamical model is also applied to the unzipping of long (kilo-basepair) DNA molecules at constant force.

引用

页码：153 / 161

页数：8