Evaluation of the discriminance capacity of RAPD, isoenzymes and morphologic markers in apple (Malus x domestica Borkh.) and the congruence among classifications

被引：18
作者：
Royo J.B. [1 ]
Itoiz R. [1 ]
机构：
[1] Dpto. Producción Agraria, Univ. Pública de Navarra, Campus Arrosadía s/n, 31006, Pamplona
来源：
Genetic Resources and Crop Evolution | 2004年 / 51卷 / 2期
关键词：
Congruence; Genetic diversity; Malus; RAPD fingerprint;
D O I：
10.1023/B:GRES.0000020857.29125.2b
中图分类号：
学科分类号：
摘要：
Twenty-one apple accessions were characterised and classified taxonomically with randomly amplified polymorphic DNA (RAPD). The results were compared with morphological and isoenzymatic classifications of the same material by calculating the congruence between similarity matrices. The results confirmed the greater discrimination capacity of RAPD compared with isoenzymes, although the taxonomic clusters obtained with both types of markers were congruent. The genetic relationships calculated using RAPD and isoenzymes showed little congruence with the morphological relationships among accessions. Isoenzymatic markers are useful in germplasm banks to evaluate the diversity of newly introduced accessions. The use of RAPD markers are especially beneficial to discriminate between material that is genetically similar, to evaluate genetic variability within a collection and to choose the components of the core collection.
引用
收藏
页码：153 / 160
页数：7
相关论文
共 35 条
  • [1] Buso G.S.C., Rangel P.H., Ferreira M.E., Analysis of genetic variability of South American wild rice populations (Oryza glumaepatula) with isoenzymes and RAPD markers, Molecular Ecology, 7, pp. 107-117, (1998)
  • [2] Chan K.F., Sun G.L., Genetic diversity and relationships detected by isozyme and RAPD analysis of crop and wild species of Amaranthus, Theoretical and Applied Genetics, 95, pp. 865-873, (1997)
  • [3] Depicker A., De Loose M., Van Bockstaele E., The role of biotechnology in plant breeding, Acta Horticulturae, 355, pp. 195-207, (1994)
  • [4] Dunemann F., Kahnau R., Schmidt H., Genetic relationships in Malus evaluated by RAPD fingerprinting of cultivars and wild species, Plant Breeding, 113, pp. 150-159, (1994)
  • [5] Foulton T.M., Chunuuongse J., Tanksley S.D., Micropep protocol for extraction of DNA from tomato and others herbaceous plants, Plant Molecular Biology Reporter, 13, pp. 207-209, (1995)
  • [6] Gardiner S.E., Bassett H.C.M., Madie C., Noiton D.A.M., Isozyme, RAPD and RFLP markers used to deduce a putative parent for the "Braeburn" apple, Journal of the American Society for Horticultural Science, 121, pp. 996-1001, (1996)
  • [7] Hamrick J.L., Isozymes and the analysis of genetic structure in plant populations, Isozymes in Plant Biology, pp. 87-105, (1989)
  • [8] Harada T., Matsukawa K., Sato T., Ishikawa R., Niizaki M., Soito K., DNA-RAPDs detec genetic variation and paternity in Malus, Euphytica, 65, pp. 87-91, (1993)
  • [9] Hemmat M., Weeden N.F., Manganaris A.G., Lawson D.M., Molecular marker linkage map in Malus, Journal of Heredity, 85, pp. 4-11, (1994)
  • [10] Descriptor List for Apple (Malus), (1982)
1234