Discrimination between some Mycoplasma spp. and Acholeplasma laidlawii in bovine milk using high resolution melting curve analysis

被引：8

作者：

Al-Farha A.A.-B. ^{[1
,2
]}

Petrovski K. ^{[1
,3
,4
]}

Jozani R. ^{[5
]}

Hoare A. ^{[6
]}

Hemmatzadeh F. ^{[1
,3
]}

机构：

[1] School of Animal and Veterinary Sciences, Roseworthy Campus, University of Adelaide, Roseworthy, 5371, SA

[2] Mosul Technical Institute, Technical Foundation, Northern Technical University, Mosul

[3] Australian Centre for Antimicrobial Resistance Ecology, University of Adelaide, Adelaide, 5000, SA

[4] Davies Centre, School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, 5371, SA

[5] Department of Veterinary Clinical Sciences, Tabriz University, Tabriz

[6] South East Vets, Mt Gambier, 5290, SA

来源：

BMC Research Notes | / 11卷 / 1期

关键词：

Acholeplasma; Cattle; Mastitis; Milk; Mycoplasma;

D O I：

10.1186/s13104-018-3223-y

中图分类号：

学科分类号：

摘要：

Objectives: This study aimed to provide a rapid, accurate and cost-effective diagnostic real time polymerase chain reaction-high resolution melting curve assay (PCR-HRM) to identify and distinguish between four different mycoplasmas and Acholeplasma laidlawii isolated at cow-level from a single commercial dairy farm in South Australia. One set of genus-level universal primers was designed targeting the 16S ribosomal RNA gene. Results: Real time PCR-HRM analysis was able to identify and distinguish between five different mollicutes, namely A. laidlawii, M. arginini, M. bovirhinis, M. bovis and uncultured Mycoplasma. Results were confirmed through sequencing. Our developed assay provides rapid and accurate screening for Mycoplasma mastitis detection. © 2018 The Author(s).

引用

共 18 条

[1]

Kirk J.H., Lauerman L.H., Roberts C., Mycoplasma mastitis in dairy cows, Compend Contin Educ Pract Vet, 16, 4, pp. 541-552, (1994)

[2]

Nicholas R., Ayling R., McAuliffe L., Mycoplasma mastitis, Vet Rec, 160, 11, (2007)

[3]

Boonyayatra S., Fox L.K., Gay J.M., Sawant A., Besser T.E., Discrimination between Mycoplasma and Acholeplasma species of bovine origin using digitonin disc diffusion assay, nisin disc diffusion assay, and conventional polymerase chain reaction, J Vet Diagn Investig, 24, 1, pp. 7-13, (2012)

[4]

Al-Farha A.A.B., Hemmatzadeh F., Khazandi M., Hoare A., Petrovski K., Evaluation of effects of Mycoplasma mastitis on milk composition in dairy cattle from South Australia, BMC Vet Res, 13, 1, (2017)

[5]

Counter D.E., A severe outbreak of bovine mastitis associated with Mycoplasma bovigenitalium and Acholeplasma laidlawii, Vet Rec, 103, 7, pp. 130-131, (1978)

[6]

Hahn R.G., Kenny G.E., Differences in arginine requirement for growth among arginine-utilizing Mycoplasma species, J Bacteriol, 117, 2, pp. 611-618, (1974)

[7]

Ghorashi S.A., Noormohammadi A.H., Markham P.F., Differentiation of Mycoplasma gallisepticum strains using PCR and high-resolution melting curve analysis, Microbiology, 156, pp. 1019-1029, (2010)

[8]

Rebelo A.R., Parker L., Cai H.Y., Use of high-resolution melting curve analysis to identify Mycoplasma species commonly isolated from ruminant, avian, and canine samples, J Vet Diagn Investig, 23, 5, pp. 932-936, (2011)

[9]

Aebi M., Van Den Borne B.H., Raemy A., Steiner A., Pilo P., Bodmer M., Mycoplasma bovis infections in Swiss dairy cattle: A clinical investigation, Acta Vet Scand, 57, (2015)

[10]

Kunkel J.R., Isolation of Mycoplasma bovis from bulk milk, Cornell Vet, 75, 3, pp. 398-400, (1985)

← 1 2 →