Recombinant E2 protein enhances protective efficacy of inactivated bovine viral diarrhea virus 2 vaccine in a goat model

被引：11
作者：
Chung Y.-C. [1 ]
Cheng L.-T. [1 ]
Zhang J.-Y. [1 ]
Wu Y.-J. [1 ]
Liu S.-S. [2 ]
Chu C.-Y. [1 ]
机构：
[1] National Pingtung University of Science and Technology, Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, 1, Shuehfu Road, Neipu, Pingtung
[2] National Pingtung University of Science and Technology, Department of Veterinary Medicine, College of Veterinary Medicine, 1, Shuehfu Road, Neipu, Pingtung
来源：
BMC Veterinary Research | / 14卷 / 1期
关键词：
BVDV; E2; Inactivated vaccine; Subunit vaccine;
D O I：
10.1186/s12917-018-1520-2
中图分类号：
学科分类号：
摘要：
Background: Inactivated and subunit bovine viral diarrhea virus (BVDV) vaccines have shown limited protective efficacy. This study aimed to evaluate the effectiveness of a vaccine containing both inactivated BVDV (iBVDV) and baculovirus-expressed recombinant E2 (rE2), an important BVDV antigen with strongly neutralizing epitopes. Results: Four groups of goats were immunized twice with one of four vaccine preparations: 1) iBVDV+rE2, 2) rE2, 3) iBVDV, and 4) saline, and challenged with BVDV. For goats vaccinated with the iBVDV+rE2 vaccine, no viremia was observed after challenge, and clinical signs, pyrexia, and leukopenia were reduced compared to the saline group. In contrast, for goats vaccinated with either iBVDV or rE2 alone, viremia was still detectable. Conclusion: The combination of iBVDV and rE2 elicited stronger protective immune responses against BVDV than iBVDV or rE2 alone. © 2018 The Author(s).
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