Application of several types of substrates to ficin-catalyzed peptide synthesis

被引：0
作者：
H. Sekizaki
E. Toyota
T. Fuchise
S. Zhou
Y. Noguchi
K. Horita
机构：
[1] Health Sciences University of Hokkaido,Faculty of Pharmaceutical Sciences
[2] Qingdao University,Pharmic Department, Medical College
来源：
Amino Acids | 2008年 / 34卷
关键词：
Keywords: ; -Boc-Amino acid – Amidinophenyl esters – Guanidinophenyl esters – (Guanidinomethyl)phenyl ester – Guanidinonaphthyl ester – Enzymatic peptide synthesis – Ficin;
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摘要：
The capability of ficin, a cystine protease, to form peptide bonds was investigated using several types of N-Boc-amino acid phenyl and naphthyl esters as acyl donor components. Enzyme-catalyzed peptide synthesis was carried out under optimized reaction conditions of pH, acyl acceptor concentration and selection of the best yield organic solvent. It used a condensation of N-Boc-Ala-OpGu and Ala-pNA as a model reaction. The products were obtained in 72–96% yield using 10 different substrates, within a few minutes of reaction time.
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