Regeneration of plants via somatic embryogenesis from leafbase and leaf tip segments ofEchinochloa colona

被引：0
作者：
S. Samantaray
G. R. Rout
P. Das
机构：
[1] Regional Plant Resource Centre,Plant Biotechnology Division, Plant Tissue Culture Laboratory
[2] Bhubaneswar,Plant Physiology and Biochemistry Laboratory
[3] Regional Plant Resource Centre,undefined
[4] Bhubaneswar,undefined
来源：
Plant Cell, Tissue and Organ Culture | 1997年 / 47卷
关键词：
gramineae; histology; somatic embryo;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Plant regeneration via somatic embryogenesis was achieved in leafbase and leaf tip explants derived from 10-day-oldin vitro-grown seedlings ofEchinochloa colona. Somatic embryogenesis was induced in the callus on Murashige and Skoog (1962) medium supplemented with 4.44 µM 6-benzyladenine, 4.64 µM kinetin and 8.05 µM 1-naphthaleneacetic acid after 4 weeks of culture incubated for 14 days in continuous dark and subsequently under a 14-h photoperiod. The incidence of somatic embryogenesis was greater in leafbase- than in leaf tip-derived calluses. Histological observations revealed various stages of development of somatic embryogenesis. The embryos matured and germinated on fresh medium lacking growth regulators. The somatic embryo-derived plantlets were established in soil.
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页码：119 / 125
页数：6
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