A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation

被引：4
作者：
Ferrante J.A. [1 ,2 ]
Giles M.R. [2 ]
Benzie E. [3 ]
Hunter M.E. [2 ]
机构：
[1] Cherokee Nation Technologies Contracted to the U.S. Geological Survey, Wetland and Aquatic Research Center, 7920 NW 71st Street, Gainesville, 32653, FL
[2] U.S. Geological Survey, Wetland and Aquatic Research Center, 7920 NW 71st Street, Gainesville, 32653, FL
[3] University of California-Davis, 1 Shields Ave, Davis, 95616, CA
来源：
BMC Research Notes | / 11卷 / 1期
关键词：
Blood; Genomic DNA; Isolation; Preservation; RNA; Tempus; Wildlife;
D O I：
10.1186/s13104-018-3671-4
中图分类号：
学科分类号：
摘要：
Objective: We use Tempus blood RNA tubes (Applied Biosystems) during health assessments of American moose (Alces alces spp.) as a minimally invasive means to obtain RNA. Here we describe a novel protocol to additionally isolate high-quality DNA from the supernatant remaining after the RNA isolation methodology. Metrics used to qualify DNA quality included measuring the concentration, obtaining a DNA integrity number from a genomic DNA ScreenTape assay (Agilent), and running the isolated DNA on an agarose gel. Results: Of the 23 samples analyzed, the average DNA concentration was 121 ng/μl (range 4-337 ng/μl) and a genomic DNA ScreenTape assay of seven samples indicated high DNA integrity values for 6 of the 7 samples (range 9.1-9.4 out of 10). Of the DNA sent for genotyping by sequencing, all proved to be of sufficient integrity to yield high-quality next-generation sequence results. We recommend this simple procedure to maximize the yield of both RNA and DNA from blood samples. © 2018 The Author(s).
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