Biotreatment of azo dye containing textile industry effluent by a developed bacterial consortium immobilised on brick pieces in an indigenously designed packed bed biofilm reactor

This study highlights the development of a lab-scale, indigenously designed; Packed-Bed Biofilm Reactor (PBBR) packed with brick pieces. The developed biofilm in the reactor was used for the decolourisation and biodegradation of the textile industry effluent. The PBBR was continuously operated for 264 days, during which 301 cycles of batch and continuous treatment were operated. In batch mode under optimised conditions, more than 99% dye decolourisation and ≥ 92% COD reduction were achieved in 6 h of contact time upon supplementation of effluent with 0.25 g L−1 glucose, 0.25 g L−1 urea, and 0.1 g L−1 phosphates. A decolourisation rate of 133.94 ADMI units h−1 was achieved in the process. PBBR, when operated in continuous mode, showed ≥ 95% and ≥ 92% reduction in ADMI and COD values. Subsequent aeration and passage through the charcoal reactor assisted in achieving a ≥ 96% reduction in COD and ADMI values. An overall increase of 81% in dye-laden effluent decolourisation rate, from 62 to 262 mg L−1 h−1, was observed upon increasing the flow rate from 18 to 210 mL h−1. Dye biodegradation was determined by UV–Vis and FTIR spectroscopy and toxicity study. SEM analysis showed the morphology of the attached-growth biofilm.