Expression and Characterization of Glucose Oxidase from Aspergillus niger in Yarrowia lipolytica

被引：0
作者：
Fatemeh Khadivi Derakshan
Farshad Darvishi
Mehrouz Dezfulian
Catherine Madzak
机构：
[1] Azad University,Department of Microbiology, Karaj Branch
[2] University of Maragheh,Microbial Biotechnology and Bioprocess Engineering (MBBE) Group, Department of Microbiology, Faculty of Science
[3] Université Paris-Saclay,UMR GMPA, AgroParisTech, INRA
来源：
Molecular Biotechnology | 2017年 / 59卷
关键词：
Glucose oxidase; Expression; Characterization;
D O I：
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中图分类号：
学科分类号：
摘要：
Glucose oxidase (GOX) is currently used in clinical, pharmaceutical, food and chemical industries. The aim of this study was expression and characterization of Aspergillus niger glucose oxidase gene in the yeast Yarrowia lipolytica. For the first time, the GOX gene of A. niger was successfully expressed in Y. lipolytica using a mono-integrative vector containing strong hybrid promoter and secretion signal. The highest total glucose oxidase activity was 370 U/L after 7 days of cultivation. An innovative method was used to cell wall disruption in current study, and it could be recommended to use for efficiently cell wall disruption of Y. lipolytica. Optimum pH and temperature for recombinant GOX activity were 5.5 and 37 °C, respectively. A single band with a molecular weight of 80 kDa similar to the native and pure form of A. niger GOX was observed for the recombinant GOX in SDS-PAGE analysis. Y. lipolytica is a suitable and efficient eukaryotic expression system to production of recombinant GOX in compered with other yeast expression systems and could be used to production of pure form of GOX for industrial applications.
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页码：307 / 314
页数：7
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