Study of gene expression of cytokines and apoptotic factors in blood and tissues from patients with surgery infection by the method of real-time polymerase chain reaction

The expression of cytokines and markers of apoptosis was studied in the whole blood of 10 volunteers by means of reverse transcription method combined with real-time PCR. These factors were also measured in the whole blood, in inflammation nodal tissues and in amputated tissues taken from 17 patients with local surgical infection. No expression of the examined factors in whole blood of volunteers has been observed. However, genes of cytokines and the apoptotic markers were expressed in different levels in lymphocytes of the whole blood of patients with local surgery infection. This expression was lower in tissues from the amputated tissues and was almost absent in tissues from the inflammation nidus, except of the gene, encoding caspase-8. These results suggest that number of pro-and antiinflammating cytokines and apoptotic factors are expressed in peripheral blood and tissue lymphocytes at the formation of the systemic inflammation response syndrome.