Stable RNA Interference in Spodoptera frugiperda Cells by a DNA Vector-based Method

被引：0

作者：

Chih-Chien Lin

John T. -A. Hsu

Kai-Ling Huang

Hung-Kuan Tang

Yiu-Kay Lai

机构：

[1] National Tsing Hua University,Department of Life Science and Institute of Biotechnology

[2] Division of Biotechnology and Pharmaceutical Research National Health Research Institutes,Department of Chemical Engineering

[3] National Tsing Hua University,undefined

来源：

Biotechnology Letters | 2006年 / 28卷

关键词：

baculovirus; double-stranded RNA; RNA interference; stable RNAi;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Double-stranded RNA (dsRNA)-mediated interference (RNAi) is a powerful tool for silencing of gene expression in many organisms. To establish a DNA vector-based method for stable RNAi in Spodoptera frugiperda cells (Sf9), we created a stably transfected Sf9 cell line to express large dsRNA fragment targeting to silence the firefly luciferase gene (luc). The luc dsRNA specifically and stably suppressed the baculovirus-mediated luciferase expression. Thus, gene silencing in Sf9 cells was achieved using DNA vectors similar to the facile design described in this study.

引用

页码：271 / 277

页数：6

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