Scanning laser ophthalmoscope imaging of fluorescein-labelled blood cells

被引：0
作者：
Jean-François Le Gargasson
Michel Paques
Jean-Eric Guez
Bernadette Boval
Eric Vicaut
Xin Hou
Yvon Grall
Alain Gaudric
机构：
[1] Hôpital Lariboisière,Biophysics Department
[2] Hôpital Lariboisière,Ophthalmology Department
[3] Hôpital Lariboisière,Banque du Sang
[4] Hôpital,INSERM U141
[5] Fernand Widal,undefined
来源：
Graefe's Archive for Clinical and Experimental Ophthalmology | 1997年 / 235卷
关键词：
Fluorescein; Bright Spot; Retinal Vessel; Particle Flow; Scanning Laser Ophthalmoscope;
D O I：
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学科分类号：
摘要：
• Purpose: To demonstrate the feasibility of a technique for the visualization by scanning laser ophthalmoscope (SLO) of fluores cein-labelled autologous leukocytes and platelets in retinal vessels. • Method: Individual blood samples from rats and rabbits were centrifuged to isolate platelets and leukocytes, then passively labelled with fluorescein and reinjected into the same animal. An SLO was used to visualize and record cell displacement in the retinal circulation. Labelled platelets were analysed by flow cytometry. • Results: By SLO, platelets appeared as a heterogeneous particle flow, and individual leukocytes appearing as brighter spots could easily be traced. Flow cytometry showed that after labelling platelets were well individualized and their size was slightly increased. • Conclusion: Circulating blood cells can be visualized in retinal vessels by a simple method consisting of passive labelling of autologous platelets and leukocytes by fluorescein. No platelet toxicity was detected. This method could be applied to the study of blood cell movement in human retinal vascular diseases.
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页码：56 / 58
页数：2
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