Genetic diversity of Leishmania major strains isolated from Phlebotomus papatasi and Phlebotomus alexandri in western Iran based on minicircle kDNA

被引：2

作者：

Khoshnood S. ^{[1
]}

Tavalla M. ^{[2
]}

Mohaghegh M.A. ^{[3
,4
]}

Khamesipour F. ^{[5
,6
]}

Hejazi S.H. ^{[1
,7
]}

机构：

[1] Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan

[2] Department of Parasitology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz

[3] Department of Laboratory Sciences, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh

[4] Health Sciences Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh

[5] Shahid Beheshti University of Medical Sciences, Tehran

[6] Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord

[7] Skin Diseases and Leishmaniasis Research Center, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan

来源：

Journal of Parasitic Diseases | 2021年 / 45卷 / 3期

关键词：

Genetic diversity; Iran; Leishmania; Minicircle kDNA; Sandflies;

D O I：

10.1007/s12639-021-01369-y

中图分类号：

学科分类号：

摘要：

This study aimed to screen the natural infection rate of Leishmania major in Phlebotomus papatasi and Phlebotomus alexandri in two counties (Mehran and Dehloran) of Ilam province as cutaneous leishmaniasis endemic areas in the west of Iran. Furthermore, the genetic diversity of parasite species that are isolated from vectors, was investigated. Sandflies were collected by sticky traps from May 2018 to October 2018. Afterward, specimens were prepared for species identification by morphological features. DNA was extracted from female sandflies, and minicircle kDNA was used to identify Leishmania isolates through nested-PCR, followed by genetic diversity between Leishmania isolates was investigated by sequence analysis of the amplified minicircle kDNA. Natural infection of the L. major was shown in all positive specimens using nested-PCR. Analysis of data from 14 isolates displayed a high level of genetic diversity in L. major. In the phylogenetic trees, all of the L. major isolates occurred in six clusters. Clusters I, II, III, and VI contained isolated strains from P. papatasi. While clusters IV and V contained isolated strains from P. alexandri. Genetic diversity of L. major isolated from vectors was investigated in western Iran for the first time. According to the results of this study, probably “various clones of L. major populations are distributed in the study area. © 2021, Indian Society for Parasitology.

引用

页码：838 / 844

页数：6

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