Responses of sweet potato peroxidases to sodium nitroprusside-mediated nitric oxide

被引：0
作者：
Yun-Hee Kim
Jae Cheol Jeong
Haeng-Soon Lee
Sang-Soo Kwak
机构：
[1] Gyeongsang National University,Department of Biology Education, College of Education, and Plant Molecular Biology and Biotechnology Research Center
[2] Korea Research Institute of Bioscience and Biotechnology (KRIBB),Plant Systems Engineering Research Center
来源：
Journal of Plant Biochemistry and Biotechnology | 2014年 / 23卷
关键词：
Nitric oxide; Peroxidase; Sweet potato; Sodium nitroprusside; Carboxyl-PTIO;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
To ascertain the response of sweetpotato peroxidases (PODs) to nitric oxide (NO), we treated the leaves of sweet potato with the NO generator sodium nitroprusside (SNP) and the NO scavenger carboxyl-PTIO (cPTIO). Exogenous application of more than 5 mM SNP caused damage to sweetpotato leaves at 24 h after treatment. The accumulation of NO in leaves was positively correlated with the SNP dose. The specific activity of PODs in sweet potato leaves was markedly increased by treatment with greater than 1 mM SNP for 24 h, whereas POD activity and accumulated NO content decreased to low levels by treatment with cPTIO. Expression analysis of POD genes in response to treatment with SNP and cPTIO revealed that major stress-inducible acidic genes, such as swpa1, swpa2, swpa3, and swpa4, were specifically regulated. These results indicate that increased NO levels in sweet potato leaves are closely linked to an improved defense capability mediated by stress-inducible PODs.
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页码：440 / 443
页数：3
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