Magnesium corrosion particles do not interfere with the immune function of primary human and murine macrophages

被引：22

作者：

Roth I. ^{[1
,2
]}

Schumacher S. ^{[1
]}

Basler T. ^{[3
]}

Baumert K. ^{[4
]}

Seitz J.-M. ^{[5
]}

Evertz F. ^{[6
]}

Müller P.P. ^{[7
]}

Bäumer W. ^{[8
]}

Kietzmann M. ^{[1
]}

机构：

[1] Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Foundation, Bunteweg 17, Hannover

[2] Elanco Animal Health, Lilly Deutschland GmbH, WernerReimers-Str. 2-4, Bad Homburg

[3] Institute of Microbiology, University of Veterinary Medicine Hannover, Foundation, Bunteweg 17, Hannover

[4] Division of Immunodermatology and Allergy Research, Clinic for Dermatology, Allergy and Venereology Hannover Medical School, Carl-Neuberg-Str. 1, Hannover

[5] Institute of Materials Science, Leibniz Universität Hannover, An der Universität 2, Garbsen, Hannover

[6] Institute of Multiphase Processes, Leibniz Universität Hannover, Callinstr. 36, Hannover

[7] Helmholtz Centre for Infection Research, Inhoffenstraße 7, Brunswick

[8] MBS Department, NCSU College of Veterinary Medicine, Research Building, Office 452, Lab 218, 1060 William Moore Drive, Raleigh, 27607, NC

来源：

Progress in Biomaterials | 2015年 / 4卷 / 1期

关键词：

Degradation; Immunotoxic; In vitro; Infection; Inflammation; Phagocytes;

D O I：

10.1007/s40204-014-0032-9

中图分类号：

学科分类号：

摘要：

Magnesium is currently under investigation as a prospective biodegradable implant material. Biodegradation of magnesium causes a release of magnesium, hydroxide ions and hydrogen gas but it can also lead to the formation of particulate debris. Implant-derived particles may have immunotoxic effects. To investigate the influence of magnesium-derived particles on the immune functions of primary macrophages, up to 500 lg/ml magnesium or magnesium corrosion particles were added to the cell culture medium. No major effects were observed on cell viability and on the release of the proinflammatory cytokine tumor necrosis factor (TNF)α. In addition, the ability of macrophages to stimulate proliferation of allogenic lymphocytes in a mixed leukocyte reaction remained unaffected. When macrophages were incubated with magnesium particles and then infected with the apathogenic Mycobacterium smegmatis, infection-induced TNFα secretion from murine macrophages was inhibited but not from human macrophages. However, the bactericidal activity of either cell type was not influenced. In conclusion, magnesiumrelated particles did not restrict the immune function of macrophages, suggesting that magnesium implants and corrosion particles derived thereof are highly biocompatible and have a low inflammatory potential. © The Author(s) 2014.

引用

页码：21 / 30

页数：9

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