Effect of phalloidin on the skeletal muscle ADP-actin filaments

被引：0

作者：

Réka Dudás

Tünde Kupi

Andrea Vig

J. Orbán

D. Lőrinczy

机构：

[1] University of Pécs,Department of Biophysics

[2] Medical School,undefined

来源：

Journal of Thermal Analysis and Calorimetry | 2009年 / 95卷

关键词：

ADP-actin filament; calorimetry; phalloidin; stability; thermodynamics;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The effect of phalloidin on the thermal stability of skeletal actin filaments polymerized from ADP-binding monomers was investigated with the method of differential scanning calorimetry. Phalloidin shifted the melting temperature of the ADP-F-actin from 59.1±1.0 to 80.0±1.2°C. The stabilizing effect of phalloidin propagated cooperatively along the filament. The cooperativity factor according to the applied model was 1.07±0.11. With these measurements it was possible to demonstrate that the binding of phalloidin has lower influence on the adjacent protomers in ADP- (k=1) than in ATP-actin filaments (k=3).

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页码：709 / 712

页数：3

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