Molecular evidence of an isolate of mungbean yellow mosaic India virus with a recombinant DNA B component occurring on mungbean from mid-hills of Meghalaya, India

被引：7

作者：

Banerjee A. ^{[1
,4
]}

Umbrey Y. ^{[2
]}

Yadav R.M. ^{[3
]}

Roy S. ^{[3
,4
]}

机构：

[1] ICAR Research Complex for NEH Region, Umiam, 793 103, Meghalaya

[2] College of Post Graduate Studies, Central Agricultural University, Umiam, 793 103, Meghalaya

[3] ICAR-National Bureau of Plant Genetic Resources, Regional Station, Umiam, 793 103, Meghalaya

[4] ICAR-National Rice Research Institute, Central Rainfed Upland Rice Research Station, Hazaribag, 825 301, Jharkhand

来源：

VirusDisease | 2018年 / 29卷 / 1期

关键词：

Genome; Mungbean; MYMIV; MYMV; Northeast India; Recombination;

D O I：

10.1007/s13337-018-0429-5

中图分类号：

学科分类号：

摘要：

A new isolate (Mg-mungbean-1) of yellow mosaic virus (YMV) was identified and characterized from mungbean growing in mid-hill condition of Meghalaya, India. Full genome of components (DNA A and DNA B; NCBI accessions number KU95030 and KU95031, respectively) of the virus were amplified through rolling circle amplification and sequenced. Both, DNA A and DNA B shared a common region (CR) with 90.4% similarity. The DNA A of Mg-mungbean-1 showed maximum (97.59%) nucleotide identity with mungbean yellow mosaic India virus (MYMIV) isolate (HF922628) reported from West Bengal, India and DNA B showed ~ 96% nucleotide identity with mungbean yellow mosaic virus (MYMV) isolates having variant DNA B. Phylogenetic tree of DNA A also identified Mg-mungbean-1 as a MYMIV. Based on DNA B the current isolate grouped with the variant Indian MYMV isolates. One recombination event in the CR of DNA B of Mg-mungbean-1 was detected, where MYMV:India:clonePB1 and MYMIV:India:cloneMBB-B31 have been identified as major and minor parents, respectively. Overall, the current study indicated occurrence of an isolate of MYMIV with a recombinant DNA B component on mungbean from mid-hills of Meghalaya, India. To the best of our knowledge this is the first molecular characterization of YMV from northeast India. © 2018, Indian Virological Society.

引用

页码：68 / 74

页数：6

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