Label-free quantification of membrane-ligand interactions using backscattering interferometry

被引：0
|
作者：
Michael M Baksh
Amanda K Kussrow
Mauro Mileni
M G Finn
Darryl J Bornhop
机构：
[1] The Scripps Research Institute,Department of Chemistry
[2] The Scripps Research Institute,Department of Molecular Biology
[3] Vanderbilt Institute for Chemical Biology,Department of Chemistry
[4] Vanderbilt University,undefined
来源：
Nature Biotechnology | 2011年 / 29卷
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摘要：
Methods to measure affinities of membrane proteins and soluble ligands are cumbersome and often rely on truncations or other modifications of the membrane protein or ligand. Baksh et al. show that backscattering interferometry is a sensitive and accurate technology for the label-free quantification of ligand–membrane receptor interactions.
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页码：357 / 360
页数：3
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