Paper-based upconversion fluorescence resonance energy transfer biosensor for sensitive detection of multiple cancer biomarkers

被引：0
作者：
Sai Xu
Biao Dong
Donglei Zhou
Ze Yin
Shaobo Cui
Wen Xu
Baojiu Chen
Hongwei Song
机构：
[1] State Key Laboratory on Integrated Optoelectronics,Department of Physics
[2] College of Electronic Science and Engineering,undefined
[3] Jilin University,undefined
[4] Dalian Maritime University,undefined
来源：
Scientific Reports | / 6卷
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摘要：
A paper-based upconversion fluorescence resonance energy transfer assay device is proposed for sensitive detection of CEA. The device is fabricated on a normal filter paper with simple nano-printing method. Upconversion nanoparticles tagged with specific antibodies are printed to the test zones on the test paper, followed by the introduction of assay antigen. Upconversion fluorescence measurements are directly conducted on the test zones after the antigen-to-antibody reactions. Furthermore, a multi-channel test paper for simultaneous detection of multiple cancer biomarkers was established by the same method and obtained positive results. The device showed high anti-interfere, stability, reproducible and low detection limit (0.89 ng/mL), moreover it is very easy to fabricate and operate, which is a promising prospect for a clinical point-of-care test.
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