Detection of androclonal variation in anther-cultured rice lines using rapds

被引：6

作者：

Afza R. ^{[1
]}

Xie J. ^{[1
]}

Francisco Javier Zapata Arias M.S. ^{[1
]}

Fundi H.K. ^{[1
]}

Lee K.-S. ^{[1
]}

Bobadilla-Mucino E. ^{[1
]}

Kodym A. ^{[1
]}

机构：

[1] Plant Breeding Unit, FAO/IAEA Agriculture and Biotechnology Laboratory, Agency's Laboratories

来源：

In Vitro Cellular & Developmental Biology - Plant | 2001年 / 37卷 / 5期

关键词：

Double hapliod; Genetic variation; Oryza sativa; Polymorhism;

D O I：

10.1007/s11627-001-0112-0

中图分类号：

学科分类号：

摘要：

Random amplified polymorphic DNA analysis was used to determine the occurrence and extent of variation in rice (Oryza sativa L.) plants regenerated from anther culture. Androclonal variation in morphologically uniform progenies was detected using 40 10-mer oligonucleotide arbitrary primers. Among 27 plants from nine anther culture-derived lines, variation was detected in three plants from two lines by two primers, namely UBC 160 and UBC 209. Primer UBC 160 amplified a polymorphic band on one of the three progenies from DH-34, while UBC 209 detected polymorphisms on two out of three progenies from line DH-58. Apart from these, the amplification products were monomorphic across all the regenerants from anther culture-derived plants. Out of 40 tested primers, no difference in the banding pattern was observed in three seed-derived plants. The significance of possible androclonal variation at the DNA level in rice doubled haploid breeding and genetic mapping is discussed.

引用

页码：644 / 647

页数：3

共 31 条

[1] Armstrong C.L., Phillips R.L., Genetic and cytogenetic variation in plants regenerated from organogenic and friable, embryogenic tissue of maize, Crop Sci., 28, pp. 363-369, (1988)
[2] Arumuganathan K., Earle E.D., Nuclear DNA content of some important plant species, Plant Mol. Biol. Rep., 9, pp. 208-218, (1991)
[3] Brettell R.I.S., Dennis E.S., Reactivation of a silent Ac following tissue cultures is associated with heritable alterations in its methylation pattern, Mol. Gen. Genet., 229, pp. 365-372, (1991)
[4] Brown P.T.H., The spectrum of molecular changes associated with somaclonal variation, IAPTC Newsletter, 66, pp. 14-25, (1991)
[5] Chu C.C., Wang C.C., Sun C.S., Hsu C., Yin K.C., Chu C.Y., Bi F.Y., Establishment of an efficient medium for anther culture of rice through comparative experiments on nitrogen sources, Sci. Sin., 18, pp. 659-668, (1975)
[6] Deumling B., Clermont L., Changes in DNA content and chromosomal size during cell culture and plant regeneration of Scilla siberica: Selective chromatin diminution in response to environmental conditions, Chromosoma, 97, pp. 439-448, (1989)
[7] Devos K.M., Gale M.D., The use of random amplified polymorphic DNA markers in wheat, Theor. Appl. Genet., 84, pp. 567-572, (1992)
[8] Edallo S., Zucchinali C., Perenzin M., Salamini F., Chromosomal variation and frequency of spontaneous mutation associated with in vitro culture and plant regeneration in maize, Maydica, 26, pp. 39-56, (1981)
[9] Fourre J.-L., Berger P., Niquet L., Andre P., Somatic embryogenesis and somaclonal variation in Norway spruce: Morphogenetic and molecular approaches, Theor. Appl. Genet., 94, pp. 159-169, (1997)
[10] Godwin I.D., Sangduen N., Kunanuvatchaidach R., Piperidis G., Adkins S.W., RAPD polymorphisms among variants and phenotypically normal rice (Oryza sativa var. indica) somaclonal progenies, Plant Cell Rep., 16, pp. 320-324, (1997)

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