Decolorization and detoxification of sulphonated azo dye Red HE7B by Bacillus sp. VUS

被引：0
作者：
Vishal V. Dawkar
Umesh U. Jadhav
Mital U. Jadhav
Anuradha N. Kagalkar
Sanjay P. Govindwar
机构：
[1] Shivaji University,Department of Biochemistry
[2] Shivaji University,Department of Microbiology
来源：
World Journal of Microbiology and Biotechnology | 2010年 / 26卷
关键词：
Decolorization; Laccase; Azo dye; Ames test; Chlorophyll;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Bacillus sp. VUS decolorized Red HE7B dye (100%) within 18 h in static anoxic conditions. A significant increase in activities of lignin peroxidase, laccase, NADH-DCIP and azo reductase was observed up to complete decolourization of RHE7B. The biodegradation was monitored by UV–Visible spectroscopy (UV–VIS), Fourier Transform Infrared (FTIR) spectroscopy and High Performance Liquid Chromatography (HPLC). The final products 4-methyl-3-(1-sulfo-ethyl)-5-([1,3,5] triazin-2-ylamino)-benzenesulfonic acid; 3-(1-sulfo-ethyl)-5-([1,3,5] triazin-2-ylamino)-benzenesulfonic acid and 3-(1,2-dihydro-[1,3,5] triazin-2-ylamino)-5-sulfomethyl-benzenesulfonic acid were characterized by gas chromatography–mass spectrometry (GC–MS). The phytotoxicity study revealed the non-toxic nature of the generated products with respect to Sorghum bicolor and Triticum aestivum. The metabolites produced after degradation increased the chlorophyll content of crop seedlings. The Ames test revealed the non-mutagenicity and non-carcinogenicity of the degraded products.
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页码：909 / 916
页数：7
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  • [1] Ames B(1973)An improved bacterial test system for the detection and classification of mutagens and carcinogens Proc Natl Acad Sci USA 70 782-786
  • [2] Lee F(2007)Sudan I induces 4 genotoxic effects and oxidative DNA damage in HepG2 cells Mutat Res 627 164-170
  • [3] Durston W(1949)Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Plant Physiol 24 1-15
  • [4] An Y(2003)Treatment of a textile dye in the anaerobic baffled reactor Water SA 29 29-134
  • [5] Jiang L(1998)Isolation of a bacterial strain with the ability to utilize the sulfonated azo compound 4-carboxy-4-sulfoazobenzene as the sole source of carbon and energy Appl Environ Microbiol 64 2315-2317
  • [6] Cao J(2004)Development and in-house validation of a liquid chromatography-electrospray-tandem mass spectrometry method for the simultaneous determination of Sudan I, Sudan II, Sudan III and Sudan IV in hot chilli products J Chromatogr A 1042 123-130
  • [7] Geng C(1994)Microbial decolorization of a reactive azo dye under anaerobic conditions Water SA 21 61-69
  • [8] Zhong L(1995)Anaerobic decolorization of reactive dyes in conventional sewage treatment processes Water SA 20 341-344
  • [9] Arnon D(1996)Treatment of exhausted dye bath effluent using anaerobic digestion: laboratory and full-scale trials Water SA 22 225-235
  • [10] Bell J(2008)Biodegradation of disperse textile dye Brown 3REL by newly isolated J Appl Microbiol 105 14-24
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