Phytochemical analysis and assessment of in vitro antibacterial activity of non-polar solvent based Camelina seed extracts

被引：6

作者：

Kumar K. ^{[1
]}

Pathak R. ^{[1
]}

机构：

[1] MBGE and Nanochemistry Laboratory, Defence Institute of Bio-Energy Research (DIBER), DRDO, Haldwani, 263139, Uttarakhand

来源：

Indian Journal of Plant Physiology | 2016年 / 21卷 / 3期

关键词：

Agar disc diffusion assay; Camelina seed; In vitro antimicrobial activity; Non-polar solvents; Phytochemicals;

D O I：

10.1007/s40502-016-0223-6

中图分类号：

学科分类号：

摘要：

A study was conducted to examine the phytochemical and antibacterial potential of non-polar solvent (hexane, petroleum ether, chloroform, and toluene) based Camelina sativa cv. Calena (EC643910) seed extracts for future investigation into the field of pharmacology, phytochemistry, ethnobotany and other biological actions for drug discovery. Among all tested extracts, hexane (disc 1) showed no inhibition zone against any pathogens, while petroleum ether based camelina seed extract (disc 2) showed sensitive activity with inhibition zones ranging from ~12 to 20 mm against most of the tested pathogens. Similarly, chloroform extract (disc 4) also showed significant activity with inhibition zones ranging from ~11 to 21 mm against all tested pathogens. Toluene extract showed no inhibition zone against Bacillus pumilus, Bacillus subtilis, Bacillus thurengenensis, Pseudomonas fluorescens, but showed significant inhibition zones ranging from ~13 to 18 mm, against Escherichia coli, Agrobacterium tumefaciens and Ralstonia solanacearum. The inhibition zones ranged from 9 to 29 mm for antibacterial activity against standard drug (disc 3 and 6). Chloroform extract showed highest phenolic (~3–4 µg g−1 dry wt), terpene (~2.6 µg of linalool g−1 dry wt), alkaloid (5 µg of atropine g−1 dry wt) and free amino acid (6.4 µg g−1 dry wt) accumulation, whereas petroleum ether extract showed highest (21.5 %) free radical scavenging property. Based on the phytochemical and antibacterial potential with different extracts, camelina was identified as the most suitable source for drug discovery. © 2016, Indian Society for Plant Physiology.

引用

页码：255 / 262

页数：7

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