Light-sheet fluorescence microscopy for quantitative biology

被引：0
|
作者：
Ernst H K Stelzer
机构：
[1] Ernst H.K. Stelzer is at the Buchmann Institute for Molecular Life Sciences,
[2] Fachbereich Lebenswissenschaften (FB15,undefined
[3] IZN),undefined
[4] Goethe Universität Frankfurt am Main,undefined
[5] Frankfurt am Main,undefined
[6] Germany.,undefined
来源：
Nature Methods | 2015年 / 12卷
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摘要：
In light sheet–based fluorescence microscopy (LSFM), optical sectioning in the excitation process minimizes fluorophore bleaching and phototoxic effects. Because biological specimens survive long-term three-dimensional imaging at high spatiotemporal resolution, LSFM has become the tool of choice in developmental biology.
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页码：23 / 26
页数：3
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