Micropropagation of commercially cultivated Henna (Lawsonia inermis) using nodal explants

被引：20

作者：

Ram K. ^{[1
]}

Shekhawat N.S. ^{[1
]}

机构：

[1] Biotechnology Unit, Department of Botany, Jai Narain Vyas University

来源：

Physiology and Molecular Biology of Plants | 2011年 / 17卷 / 3期

关键词：

Ex vitro rooting; In vitro propagation; Lawsonia inermis; Plant growth regulator; Repeated transfer;

D O I：

10.1007/s12298-011-0069-3

中图分类号：

学科分类号：

摘要：

Lawsonia inermis Linn. (Mehandi) is cultivated as cash crop in India particularly in Sojat area of Pali district, Rajasthan. Present investigation describes an efficient regeneration system for elite genotype of L. inermis using nodal segments. Optimum response in terms of percent cultures responding, days to bud break and average shoot length was observed on MS medium supplemented with 6-benzylaminopurine (BA; 2.0 mg l-1). Shoot multiplication was influenced by plant growth regulators, repeated transfer of explants and addition of ammonium sulphate. Maximum shoots were regenerated on MS medium supplemented with BA (0.25 mg l-1), kinetin (Kn; 0.25 mg l-1), indole-3-acetic acid (IAA; 0.1 mg l-1) and ammonium sulphate (150 mg l-1). To reduce resources, time and labours costs, we have also attempted ex vitro rooting of shoots. About 95 % shoots were rooted ex vitro on soilrite after treatment with indole-3-butyric acid (IBA; 300 mg l-1) and 2-naphthoxy acetic acid (NOA; 100 mg l-1) and establishment in soil successfully. © 2011 Prof. H.S. Srivastava Foundation for Science and Society.

引用

页码：281 / 289

页数：8

共 31 条

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