Untargeted metabolomics unveil alterations of biomembranes permeability in human HaCaT keratinocytes upon 60 GHz millimeter-wave exposure

被引:0
|
作者
Pierre Le Pogam
Yann Le Page
Denis Habauzit
Mickael Doué
Maxim Zhadobov
Ronan Sauleau
Yves Le Dréan
David Rondeau
机构
[1] Univ Rennes,Département de Chimie
[2] CNRS,undefined
[3] IETR (Institut d’Électronique et de Télécommunication de Rennes),undefined
[4] UMR 6164,undefined
[5] Univ Rennes,undefined
[6] Inserm,undefined
[7] EHESP,undefined
[8] Irset (Institut de recherche en santé,undefined
[9] environnement et travail) – UMR_S 1085,undefined
[10] Université de Bretagne Occidentale,undefined
[11] 6 avenue Victor Le Gorgeu,undefined
来源
Scientific Reports | / 9卷
关键词
D O I
暂无
中图分类号
学科分类号
摘要
A joint metabolomic and lipidomic workflow is used to account for a potential effect of millimeter waves (MMW) around 60 GHz on biological tissues. For this purpose, HaCaT human keratinocytes were exposed at 60.4 GHz with an incident power density of 20 mW/cm², this value corresponding to the upper local exposure limit for general public in the context of a wide scale deployment of MMW technologies and devices. After a 24h-exposure, endo- and extracellular extracts were recovered to be submitted to an integrative UPLC-Q-Exactive metabolomic and lipidomic workflow. R-XCMS data processing and subsequent statistical treatment led to emphasize a limited number of altered features in lipidomic sequences and in intracellular metabolomic analyses, whatever the ionization mode (i.e 0 to 6 dysregulated features). Conversely, important dysregulations could be reported in extracellular metabolomic profiles with 111 and 99 frames being altered upon MMW exposure in positive and negative polarities, respectively. This unexpected extent of modifications can hardly stem from the mild changes that could be reported throughout transcriptomics studies, leading us to hypothesize that MMW might alter the permeability of cell membranes, as reported elsewhere.
引用
收藏
相关论文
共 9 条
  • [1] Untargeted metabolomics unveil alterations of biomembranes permeability in human HaCaT keratinocytes upon 60 GHz millimeter-wave exposure
    Le Pogam, Pierre
    Le Page, Yann
    Habauzit, Denis
    Doue, Mickael
    Zhadobov, Maxim
    Sauleau, Ronan
    Le Drean, Yves
    Rondeau, David
    SCIENTIFIC REPORTS, 2019, 9 (1)
  • [2] Untargeted Metabolomics Reveal Lipid Alterations upon 2-Deoxyglucose Treatment in Human HaCaT Keratinocytes
    Le Pogam, Pierre
    Doue, Mickael
    Le Page, Yann
    Habauzit, Denis
    Zhadobov, Maxim
    Sauleau, Ronan
    Le Drean, Yves
    Rondeau, David
    JOURNAL OF PROTEOME RESEARCH, 2018, 17 (03) : 1146 - 1157
  • [3] ACUTE OCULAR INJURIES CAUSED BY 60-GHZ MILLIMETER-WAVE EXPOSURE
    Kojima, Masami
    Hanazawa, Masahiro
    Yamashiro, Yoko
    Sasaki, Hiroshi
    Watanabe, Soichi
    Taki, Masao
    Suzuki, Yukihisa
    Hirata, Akimasa
    Kamimura, Yoshitsugu
    Sasaki, Kazuyuki
    HEALTH PHYSICS, 2009, 97 (03): : 212 - 218
  • [4] Dependence on Spot Size and Exposure Duration to Thermal Sensation by Millimeter-Wave Exposure at 60 GHz
    Wake, Kanako
    Soichi, Watanabe
    Nishikata, Atsuhiro
    Enomoto, Hiroyuki
    Ugawa, Yoshikazu
    PROCEEDINGS OF 2013 URSI INTERNATIONAL SYMPOSIUM ON ELECTROMAGNETIC THEORY (EMTS), 2013, : 540 - +
  • [5] Millimeter wave exposure reverses TPA suppression of gap junction intercellular communication in HaCaT human keratinocytes
    Chen, Q
    Zeng, QL
    Lu, DQ
    Chiang, H
    BIOELECTROMAGNETICS, 2004, 25 (01) : 1 - 4
  • [6] Effects of long-term exposure to 60 GHz millimeter-wave on genotoxicities in cells derived from human eye
    Koyama, Shin
    Narita, Eijiro
    Suzuki, Yukihisa
    Shiina, Takeo
    Taki, Masao
    Shinohara, Naoki
    Miyakoshi, Junji
    2014 XXXITH URSI GENERAL ASSEMBLY AND SCIENTIFIC SYMPOSIUM (URSI GASS), 2014,
  • [7] 60-GHz Millimeter-Wave Life Detection System (MLDS) for Noncontact Human Vital-Signal Monitoring
    Chuang, Huey-Ru
    Kuo, Hsin-Chih
    Lin, Fu-Ling
    Huang, Tzuen-Hsi
    Kuo, Chi-Shin
    Ou, Ya-Wen
    IEEE SENSORS JOURNAL, 2012, 12 (03) : 602 - 609
  • [8] Development of a Numerical Model of 60 GHz Band Lens Antenna for the Study of Thermo-Physiological and Cellular Function Changes Induced by Millimeter-Wave Exposure
    Yamamoto, Kohei
    Hikage, Takashi
    Masuda, Hiroshi
    Ishitake, Tatsuya
    Li, Kun
    Nagai, Akiko
    2022 INTERNATIONAL SYMPOSIUM ON ANTENNAS AND PROPAGATION (ISAP), 2022, : 277 - 278
  • [9] Scalable Modeling of Human Blockage at Millimeter-Wave: A Comparative Analysis of Knife-Edge Diffraction, the Uniform Theory of Diffraction, and Physical Optics Against 60 GHz Channel Measurements
    Mukherjee, Swagato
    Skidmore, Gregory
    Chawla, Tarun
    Bhardwaj, Anmol
    Gentile, Camillo
    Senic, Jelena
    IEEE ACCESS, 2022, 10 : 133643 - 133654