Expression of Functional Interleukin-12 from Mouse in Transgenic Tomato Plants

被引：0
|
作者：
Abel Gutiérrez-Ortega
Claudia Sandoval-Montes
Teresa Jesús de Olivera-Flores
Leopoldo Santos-Argumedo
Miguel Ángel Gómez-Lim
机构：
[1] Centro de Investigación y de Estudios Avanzados del I.P.N.,Departamento de Ingeniería Genética de Plantas
[2] Centro de Investigación y de Estudios Avanzados del I.P.N.,Departamento de Biomedicina Molecular
[3] Universidad Nacional Autónoma de México,Facultad de Química, Conjunto E, Laboratorio 116, Paseo de la Investigación Científica
来源：
Transgenic Research | 2005年 / 14卷
关键词：
interferon gamma; interleukin-12; recombinant proteins; tomato; transgenic plants;
D O I：
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学科分类号：
摘要：
Transgenic plants have been employed successfully as a low-cost system for the production of therapeutically valuable proteins, including antibodies, antigens and hormones. Here, we report the expression of a cytokine with immunomodulatory function, mouse interleukin-12 (IL-12), in transgenic tomato plants. Single-chain mouse IL-12 driven by the CaMV 35S promoter, accumulates to high levels in leaves and fruits (up to 7.3 and 3.4 μg per gram of fresh weight, respectively). Mouse IL-12 expressed in tomato displays biological activity in vitro, as determined by interferon-γ (IFN-γ) secretion by T cells. Possible uses of this plant-based cytokine involving mucosal delivery are discussed
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页码：877 / 885
页数：8
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