The effects of murine cytomegalovirus on the maturation, fertilization, cleavage and blastula formation of mouse oocytes in vitro

被引：0
|
作者：
Xinrong Wang
Xinhong Zhang
Suhua Chen
Guijin Zhu
Jihui Ai
机构：
[1] Huazhong University of Science and Technology,Department of Gynecology & Obstetrics
[2] Tongji Hospital,Reproduction Center
[3] Huazhong University of Science and Technology,undefined
来源：
Journal of Huazhong University of Science and Technology | 2007年 / 27卷
关键词：
mouse cytomegalovirus; fertilization; cleavage; blastula formation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
To study the effects of mouse cytomegalovirus (MCMV) on the in vitro maturation, fertilization, cleavage and blastula formation of mouse oocytes, the immature oocytes were infected in vitro by MCMVs of different dosages (100 TCID50, 10 TCID50 and 1 TCID50). The oocytes were then observed for in vitro maturation, fertilization, cleavage and blastula formation and the ultrastructural changes after the culture with the viruses. Our results showed that no significant differences were found in IVM, IVF, cleavage and blastula formation among the groups treated with of virus of various dosages. And ultrastructural abnormality was observed in the oocytes treated by 100 TCID50 of viruses. It is concluded that MCMV did not have any conspicuous effects on IVM, IVF, cleavage and blastula formation of murine immature oocytes.
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页码：468 / 470
页数：2
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