Analysis of glycans in glycoproteins by diffusion-ordered nuclear magnetic resonance spectroscopy

被引：0
|
作者：
Karin Ortner
Veeramuthu N. Sivanandam
Wolfgang Buchberger
Norbert Müller
机构：
[1] Johannes Kepler University,Institute of Analytical Chemistry
[2] Johannes Kepler University,Institute of Organic Chemistry
来源：
Analytical and Bioanalytical Chemistry | 2007年 / 388卷
关键词：
Nuclear magnetic resonance spectroscopy; Glycoproteins; Glycans; Diffusion;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Enzymatically cleaved glycans from sub-milligram quantities of erythropoietin (EPO) and ovalbumin have been analyzed, without further purification, by two-dimensional diffusion-ordered nuclear magnetic resonance spectroscopy. At NMR sample concentrations below 50 μmol L−1 the major components of the oligosaccharide fractions could be distinguished by their anomeric proton chemical shift and their size-dependent diffusion coefficients.
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页码：173 / 177
页数：4
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