G0/G1 Phase Arrest and Apoptosis Induced by Manganese Chloride on Cultured Rat Astrocytes and Protective Effects of Riluzole

被引：2
作者：
Yu Deng
Donghui Xu
Bin Xu
Zhaofa Xu
Yawen Tian
Wanyu Feng
Wei Liu
Haibo Yang
机构：
[1] China Medical University,Department of Environmental Health, School of Public Health
[2] First Affiliated Hospital of China Medical University,Department of Pharmacy
来源：
Biological Trace Element Research | 2011年 / 144卷
关键词：
Manganese chloride; Cell cycle; Apoptosis; Astrocytes; Riluzole;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Occupational or environmental exposure to excessive Mn would cause manganism, which is resembled Parkinson disease. However, the mechanism underlying manganism is still unknown. It had been documented that astrocytes play important roles in physiological function in brain. Therefore, in the present study, the cultured astrocytes were exposed to 0, 125, 250, and 500 μM MnCl2, and cell viability, lactate dehydrogenase (LDH) leakage, morphological change, cell cycle progression, and apoptosis were determined. In addition, 100 μM riluzole (a glutamatergic modulator) was pretreated for 6 h before no MnCl2 exposure or 500 μM MnCl2 exposure. The results showed that cell viability inhibited, LDH leakage elevated, morphology injured, G0/G1 phase cell cycle arrested, and apoptosis rate increased in a concentration-dependent manner. Further investigation indicated that riluzole pretreatment reversed cytotoxicity, cell cycle aberration, and apoptosis on astrocytes caused by MnCl2. These results suggested that MnCl2 could cause cytotoxicity, cell cycle arrest, and apoptosis concentration-dependently; riluzole might antagonize Mn toxicity on astrocytes.
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页码：832 / 842
页数：10
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