The diagnostic value of circulating microRNA-499 versus high-sensitivity cardiac troponin T in early diagnosis of ST segment elevation myocardial infarction

Background: Ischemic heart disease is the single most common cause of death worldwide. In order to increase the accuracy of the diagnosis, new diagnostic methods have been introduced, including miRNA-499, which is a promising biomarkers in coronary atherosclerotic diseases (CAD), and acute myocardial infarction (AMI). Aim of the work: To evaluate the diagnostic value of circulating miRNA-499 versus high-sensitivity cardiac troponin in early diagnosis of AMI with ST segment elevation within the first 6 h of complaint. Subjects and methods: This study was conducted on 100 Egyptian patients who presented during the first 3 h of AMI in the emergency department and 98 healthy volunteers as a control group. Serum samples for both groups were analyzed using reverse transcription and real-time PCR by TaqMan® MicroRNA assays. Results: The concentration of miRNA-499 was significantly elevated in STEMI patients compared to healthy controls (p < 0.001). Moreover, receiver operating characteristic (ROC) curve analysis showed that area under the curve (AUC) for miRNA-499 was 0.90 (95% CI, 0.845–0.955) with 89% sensitivity and 83% specificity, at which positive predictive value (PPV) was 94% andnegative predictive value (NPV) was 69%. Furthermore, miRNA-499 displayed significant positive correlation with hs-cTnT and higher expression than hs-cTnT in the first 3 h of chest pain in emergency department. Conclusion: miRNA-499 is a promising reliable biomarker for early detection of STEMI patients. © 2021, The Author(s), under exclusive licence to Springer-Verlag London Ltd. part of Springer Nature.