In vitro differentiation and plant regeneration of Albizia chinensis (Osb.) Merr.

被引：25

作者：

Sinha R.K. ^{[1
]}

Majumdar K. ^{[1
]}

Sinha S. ^{[1
]}

机构：

[1] Cytogenetics/Plant Tissue Cult. Lab., Department of Life Sciences, Tripura University, Agartala-799004, Tripura

来源：

In Vitro Cellular & Developmental Biology - Plant | 2000年 / 36卷 / 5期

关键词：

Albizia chinensis (Osb.) Merr; Cytokinin; In vitro differentiation; Plant regeneration;

D O I：

10.1007/s11627-000-0066-7

中图分类号：

学科分类号：

摘要：

Petiolar and distal cotyledonary segments (PCS and DCS) of Albizia chinensis were cultured on Murashige and Skoog's (MS; 1962) medium and induced to form adventitious shoot buds in the presence of either cytokinins 6-benzylamino purine (BAP), kinetin (KN) or thidiazuron (TDZ). Superiority of BAP in inducing shoot bud and differentiation was observed. PCS was more morphogenic to shoot bud differentiation than DCS. TDZ was highly effective in inducing shoot buds, but arrested shoot growth, while KN produced more callus during differentiation of shoots. Rapid and high rate of shoot multiplication per explant was achieved through subculture in MS medium containing BAP (1.0 mg l-1) and indole-3-acetic acid (IAA) (0.5 mg l-1). BAP at low concentration was required to enhance shoot multiplication and elongation. Successful rooting of regenerated shoots was carried out in a two-step culture procedure in MS media with indole-3-butyric acid (IBA) (2.0 mg l-1) and subsequent subculture in IBA-free medium.

引用

页码：370 / 373

页数：3

共 25 条

[1]

Bhardwaj S.D., Kumar D., Parmar Y.S., Time of sowing influences Albizia chinensis and A. lebbeck seedling growth, Nitrogen Tree Res. Rep., 12, pp. 44-46, (1994)

[2]

Biondi S., Thorpe T.A., Clonal propagation of forest tree species, pp. 197-204, (1981)

[3]

Bonga J.M., Vegetative propagation in relation to juvenility, maturity and rejuvenation, pp. 4-35, (1982)

[4]

D'Amato F., Cytogenetics of differentiation in tissue and cell cultures, pp. 343-357, (1977)

[5]

Huetteman C.A., Preece J.E., Thidiazuron: A potent cytokinin for woody plant tissue culture, Plant Cell Tiss. Organ Cult., 33, pp. 105-119, (1993)

[6]

Larkin T.J., Scowcroft W.R., Somaclonal variation - A novel source of variability from cell cultures for plant improvement. A review, Theor. Appl. Genet., 60, pp. 97-214, (1981)

[7]

Mascarenhas A.F., Gupta R.K., Kulkarni V.M., Mehta U., Iyer R.S., Khuspe S.S., Jagannathan V., Propagation of trees by tissue culture, pp. 175-179, (1981)

[8]

Murashige T., Skoog F., A revised medium for rapid growth and bioassay with tobacco tissue cultures, Physiol. Plant, 15, pp. 473-497, (1962)

[9]

Phukan M.K., Mitra G.C., In vitro regeneration of Albizia odoratissima Benth, a shade tree for the tea plantation of north east India, Two and a Bud, 30, pp. 54-58, (1983)

[10]

Pierik R.L.M., Rejuvenation and micropropagation, pp. 91-101, (1990)

← 1 2 3 →