Life span extensions associated with upregulation of gene expression of antioxidant enzymes in Caenorhabditis elegans; Studies of mutation in the age-1, PI3 kinase homologue and short-term exposure to hyperoxia

被引：9

作者：

Honda Y. ^{[1
]}

Honda S. ^{[1
]}

机构：

[1] Tokyo Metropol. Inst. of Gerontology, Tokyo, 173-0015, 35-2 Sakaecho, Itabashiku

来源：

Journal of the American Aging Association | 2002年 / 25卷 / 1期

关键词：

Antioxidant Enzyme; Catalase; Life Span; Antioxidant Defense; Insulin Receptor;

D O I：

10.1007/s11357-002-0003-2

中图分类号：

学科分类号：

摘要：

Life span could be modified by genetic or environmental perturbations in Caenorhabditis elegans. Here we show that two extensions of life span are associated with oxidative stress resistance and upregulation of the gene expression of antioxidant enzymes. First, mutations in age-1 gene (PI3 kinase homologue) that confer life span extension, display oxidative stress resistance and increase in the gene expression of sod-3, one of two Mn-superoxide dismutases (SOD) and ctl-1, cytosolic catalase. In this study, these traits appear to be regulated by the following genetic pathway: daf-2 (insulin receptor family) → daf-18 (PTEN homologue) → age-1 → daf-16 (Fork head transcription factor family), similar to the genetic pathway for the life span extension. Second, we show that short-term exposure to hyperoxia extends life span slightly but significantly. This treatment increases oxidative stress resistance and the gene expression of three types of SOD isoforms. These results suggest that both of these two life span extensions are closely related with increase in the antioxidant defense function.

引用

页码：21 / 28

页数：7

共 50 条

[1]

Weindruch R.H., Walford R.L., Fligiel S., Guthrie D., The retardation of aging in mice by dietary restriction: Longevity, cancer, immunity, and lifetime energy intake, J. Nutr., 116, pp. 641-654, (1986)

[2]

Rockstein M., Chesky J.A., Susman M.L., Comparative biology and evolution of aging, The Handbook of the Biology of Aging, pp. 3-34, (1977)

[3]

Honda S., Ishii N., Suzuki K., Matsuo M., Oxygen-dependent perturbation of life span and aging rate in the nematode, J. Gerontol., 48, (1993)

[4]

Guarente L., Kenyon C., Genetic pathways that regulate ageing in model organisms, Nature, 408, pp. 255-262, (2000)

[5]

Kimura K.D., Tissenbaum H.A., Liu Y., Ruvkun G., daf-2, An insulin receptor-like gene that regulates longevity and diapause in Caenorhabditis elegans, Science, 277, pp. 942-946, (1997)

[6]

Morris J.Z., Tissenbaum H.A., Ruvkun G.A., A phosphatidylinositol-3-OH kinase family member regulating longevity and diapause in Caenorhabditis elegans, Nature, 382, pp. 536-539, (1996)

[7]

Paradis S., Ailion M., Toker A., Thomas J.H., Ruvkun G.A., PDK1 homolog is necessary and sufficient to transduce AGE-1 PI3 kinase signals that regulate diapause in Caenorhabditis elegans, Genes Dev., 13, pp. 1438-1452, (1999)

[8]

Kenyon C., Chang J., Gensch E., Rudner A., Tabtiang R., A C. elegans mutant that lives twice as long as wild type, Nature, 366, pp. 461-464, (1993)

[9]

Lin K., Dorman J.B., Rodan A., Kenyon C., daf-16: An HNF-3/forkhead family member that can function to double the life-span of Caenorhabditis elegans, Science, 278, pp. 1319-1322, (1997)

[10]

Ogg S., Paradis S., Gottlieb S., Patterson G.I., Lee L., Tissenbaum H.A., Ruvkun G., The Fork head transcription factor DAF-16 transduces insulin-like metabolic and longevity signals in C. elegans, Nature, 389, pp. 994-999, (1997)

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